Source:http://linkedlifedata.com/resource/pubmed/id/10587472
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rdf:type | |
lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0031809,
umls-concept:C0162326,
umls-concept:C0205265,
umls-concept:C0450254,
umls-concept:C0997300,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1527178,
umls-concept:C1555582,
umls-concept:C1705938,
umls-concept:C1880371,
umls-concept:C1960936
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pubmed:issue |
2
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pubmed:dateCreated |
2000-2-16
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pubmed:abstractText |
A total of 1000 expressed sequence tags (ESTs) corresponding to 760 unique sequence sets were identified using random sequencing of clones from a cDNA library constructed from mycelial RNA of Phytophthora infestans. A number of software programs, represented by a relational database and an analysis pipeline, were developed for the automated analysis and storage of the EST sequence data. A set of 419 nonredundant sequences, which correspond to a total of 632 ESTs (63.2%), were identified as showing significant matches to sequences deposited in public databases. A putative cellular identity and role was assigned to all 419 sequences. All major functional categories were represented by at least several ESTs. Four novel cDNAs containing sequences related to elicitins, a family of structurally related proteins that induce the hypersensitive response and condition avirulence of P. infestans on Nicotiana plants, were among the most notable genes identified. Two of these elicitin-like cDNAs were among the most abundant cDNAs examined. The set also contained several ESTs with high sequence similarity to unique plant genes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Algal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/elicitin, Phytophthora
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1087-1845
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
94-106
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10587472-Actins,
pubmed-meshheading:10587472-Algal Proteins,
pubmed-meshheading:10587472-Algorithms,
pubmed-meshheading:10587472-DNA, Complementary,
pubmed-meshheading:10587472-Expressed Sequence Tags,
pubmed-meshheading:10587472-Fungal Proteins,
pubmed-meshheading:10587472-Gene Library,
pubmed-meshheading:10587472-Genetic Variation,
pubmed-meshheading:10587472-Phylogeny,
pubmed-meshheading:10587472-Phytophthora,
pubmed-meshheading:10587472-Plants, Toxic,
pubmed-meshheading:10587472-Sequence Analysis, DNA,
pubmed-meshheading:10587472-Software,
pubmed-meshheading:10587472-Tobacco
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pubmed:year |
1999
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pubmed:articleTitle |
Initial assessment of gene diversity for the oomycete pathogen Phytophthora infestans based on expressed sequences.
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pubmed:affiliation |
Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, 1680 Madison Avenue, Wooster, Ohio 44691, USA.
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pubmed:publicationType |
Journal Article
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