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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-1-12
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pubmed:abstractText |
We have applied the perforated patch whole-cell technique to beta cells within intact pancreatic islets to identify the current underlying the glucose-induced rhythmic firing of action potentials. Trains of depolarizations (to simulate glucose-induced electrical activity) resulted in the gradual (time constant: 2.3 s) development of a small (<0.8 nS) K(+) conductance. The current was dependent on Ca(2+) influx but unaffected by apamin and charybdotoxin, two blockers of Ca(2+)-activated K(+) channels, and was insensitive to tolbutamide (a blocker of ATP-regulated K(+) channels) but partially (>60%) blocked by high (10-20 mM) concentrations of tetraethylammonium. Upon cessation of electrical stimulation, the current deactivated exponentially with a time constant of 6.5 s. This is similar to the interval between two successive bursts of action potentials. We propose that this Ca(2+)-activated K(+) current plays an important role in the generation of oscillatory electrical activity in the beta cell.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-10049324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-10400636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-10414306,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-1719424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-1720583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-2072734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-2201760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-2201761,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-2407553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-2417125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-2427706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-2459299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-3056403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-381635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-4873864,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-6386515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-7473686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-8145165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-8182038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-8507191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-8643546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-8770032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-8900404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-9051586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-9575286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-9600932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-9625872,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-9657965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10578013-9804423
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Hypoglycemic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/KATP Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Large-Conductance...,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels...,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Channels, Inwardly...,
http://linkedlifedata.com/resource/pubmed/chemical/Tolbutamide,
http://linkedlifedata.com/resource/pubmed/chemical/uK-ATP-1 potassium channel
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1295
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
759-70
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10578013-ATP-Binding Cassette Transporters,
pubmed-meshheading:10578013-Action Potentials,
pubmed-meshheading:10578013-Animals,
pubmed-meshheading:10578013-Electrophysiology,
pubmed-meshheading:10578013-Hypoglycemic Agents,
pubmed-meshheading:10578013-Islets of Langerhans,
pubmed-meshheading:10578013-KATP Channels,
pubmed-meshheading:10578013-Large-Conductance Calcium-Activated Potassium Channels,
pubmed-meshheading:10578013-Membrane Potentials,
pubmed-meshheading:10578013-Mice,
pubmed-meshheading:10578013-Patch-Clamp Techniques,
pubmed-meshheading:10578013-Potassium Channel Blockers,
pubmed-meshheading:10578013-Potassium Channels,
pubmed-meshheading:10578013-Potassium Channels, Calcium-Activated,
pubmed-meshheading:10578013-Potassium Channels, Inwardly Rectifying,
pubmed-meshheading:10578013-Tolbutamide
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pubmed:year |
1999
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pubmed:articleTitle |
Activation of Ca(2+)-dependent K(+) channels contributes to rhythmic firing of action potentials in mouse pancreatic beta cells.
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pubmed:affiliation |
Department of Physiological Sciences, Division of Molecular and Cellular Physiology, Lund University, SE-223 62 Lund, Sweden.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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