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rdf:type |
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lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0039194,
umls-concept:C0205419,
umls-concept:C0851285,
umls-concept:C1155065,
umls-concept:C1512692,
umls-concept:C1515021,
umls-concept:C1704711,
umls-concept:C1709059
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pubmed:issue |
11
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pubmed:dateCreated |
1999-12-20
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pubmed:abstractText |
ADP-ribosylation of membrane proteins on mouse T cells by ecto-ADP-ribosyltransferase(s) (ARTs) can down-regulate proliferation and function. The lack of mAbs against mouse ARTs has heretofore prevented analysis of ART expression on T cell subsets. Using gene gun technology, we immunized a Wistar rat with an Art2b expression vector and produced a novel mAb, Nika102, specific for ART2.2, the Art2b gene product. We show that ART2.2 is expressed as a GPI-anchored protein on the surface of mature T cells. Inbred strain-dependent differences in ART2.2 expression levels were observed. C57BL/6J and C57BLKS/J express the Ag at high level, with up to 70% of CD4+ and up to 95% of CD8+ peripheral T cells expressing ART2.2. CBA/J and DBA/2J represent strains with lowest expression levels. T cell-deficient mice and NZW/LacJ mice with a defective structural gene for this enzyme were ART2.2 negative. In the thymus, ART2.2 expression is restricted to subpopulations of mature cells. During postnatal ontogeny, increasing percentages of T cells express ART2.2, reaching a peak at 6-8 wk of age. Interestingly, ART2.2 and CD25 are reciprocally expressed: activation-induced up-regulation of CD25 is accompanied by loss of ART2.2 from the cell surface. Nika102 thus defines a new differentiation/activation marker of thymic and postthymic T cells in the mouse and should be useful for further elucidating the function of the ART2.2 cell surface enzyme.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP Ribose Transferases,
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/Art2a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Art2b protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/FLAG peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol...,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6014-22
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pubmed:dateRevised |
2009-9-7
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pubmed:meshHeading |
pubmed-meshheading:10570289-ADP Ribose Transferases,
pubmed-meshheading:10570289-Animals,
pubmed-meshheading:10570289-Antibodies, Monoclonal,
pubmed-meshheading:10570289-Antibody Specificity,
pubmed-meshheading:10570289-Antigens, Differentiation, T-Lymphocyte,
pubmed-meshheading:10570289-Down-Regulation,
pubmed-meshheading:10570289-Histocompatibility Antigens,
pubmed-meshheading:10570289-Lymphocyte Activation,
pubmed-meshheading:10570289-Membrane Glycoproteins,
pubmed-meshheading:10570289-Membrane Proteins,
pubmed-meshheading:10570289-Mice,
pubmed-meshheading:10570289-Mice, Inbred Strains,
pubmed-meshheading:10570289-Peptides,
pubmed-meshheading:10570289-Phosphatidylinositol Diacylglycerol-Lyase,
pubmed-meshheading:10570289-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:10570289-Rats,
pubmed-meshheading:10570289-Recombinant Proteins,
pubmed-meshheading:10570289-T-Lymphocyte Subsets,
pubmed-meshheading:10570289-Thymus Gland,
pubmed-meshheading:10570289-Type C Phospholipases
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pubmed:year |
1999
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pubmed:articleTitle |
A new monoclonal antibody detects a developmentally regulated mouse ecto-ADP-ribosyltransferase on T cells: subset distribution, inbred strain variation, and modulation upon T cell activation.
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pubmed:affiliation |
Institute for Immunology, University Hospital, Hamburg, Germany; The Jackson Laboratory, Bar Harbor, ME 04609, USA. nolte@uke.uni-hamburg.de
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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