Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
1999-12-9
pubmed:abstractText
Denatured or acellular muscle grafts are known to support axonal regeneration. With increasing gap length, failure of regeneration is evident, due to the lack of viable Schwann cells in the graft. The authors created a biologic nerve conduit, in a rat sciatic nerve model, by implanting cultured Schwann cells into an acellular gracilis muscle. Autologous nerve grafts and acellular muscle grafts without Schwann cells served as controls. After 6 weeks, regeneration was assessed clinically, histologically, and morphometrically. Polymerase chain reaction (PCR) analysis showed that the implanted Schwann cells remained viable within the graft. Good regeneration was noted in the muscle-Schwann cell group, while regeneration in the muscle grafts without Schwann cells was significantly impaired. The muscle-Schwann cell graft demonstrated systematic and organized regeneration, including the proper orientation of regenerated fibers. The number of axons regenerating through the muscle-Schwann cell grafts was significantly increased, compared with the acellular muscle without Schwann cells. Implantation of Schwann cells into acellular muscle thus provided a biologic conduit with large basal lamina tubes, as a pathway for regenerating axons. The positive effects of Schwann cells, producing neurotrophic and neurotropic factors, supported axonal regeneration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0743-684X
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
531-7
pubmed:dateRevised
2011-2-16
pubmed:meshHeading
pubmed-meshheading:10566583-Animals, pubmed-meshheading:10566583-Base Sequence, pubmed-meshheading:10566583-Disease Models, Animal, pubmed-meshheading:10566583-Graft Survival, pubmed-meshheading:10566583-Male, pubmed-meshheading:10566583-Molecular Sequence Data, pubmed-meshheading:10566583-Muscle, Skeletal, pubmed-meshheading:10566583-Nerve Regeneration, pubmed-meshheading:10566583-Neural Conduction, pubmed-meshheading:10566583-Peripheral Nervous System Diseases, pubmed-meshheading:10566583-Polymerase Chain Reaction, pubmed-meshheading:10566583-Rats, pubmed-meshheading:10566583-Rats, Wistar, pubmed-meshheading:10566583-Reference Values, pubmed-meshheading:10566583-Schwann Cells, pubmed-meshheading:10566583-Sciatic Nerve, pubmed-meshheading:10566583-Sensitivity and Specificity, pubmed-meshheading:10566583-Statistics, Nonparametric
pubmed:year
1999
pubmed:articleTitle
Acellular muscle with Schwann-cell implantation: an alternative biologic nerve conduit.
pubmed:affiliation
Department of Plastic, Reconstructive, and Hand Surgery, University of Magdeburg, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't