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pubmed-article:10565024pubmed:abstractTextMethods for the selection and characterization of antisense oligonucleotides for specifically eliminating closely related gene family members are available. High-throughput semiautomated methods using 96-well plate formats and array technology and improved assays are under active development that will streamline many steps and will likely merge. Second-generation 20-mer antisense phosphorothioate oligonucleotides containing 2'-methoxyethyl groups at the first and last 6 nucleotides with improved nuclease resistance and RNA affinity are becoming available.lld:pubmed
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pubmed-article:10565024pubmed:articleTitleAntisense methods for discrimination of phenotypic properties of closely related gene products: Jun kinase family.lld:pubmed
pubmed-article:10565024pubmed:affiliationSidney Kimmel Cancer Center, San Diego, California 92121, USA.lld:pubmed
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