Source:http://linkedlifedata.com/resource/pubmed/id/10555354
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rdf:type | |
lifeskim:mentions | |
pubmed:dateCreated |
1999-12-14
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pubmed:databankReference | |
pubmed:abstractText |
Eubacterium lentum has unique phenotypic characters within the genus Eubacterium. The 16S rRNA sequence of Eubacterium lentum was determined and its phylogenetic position was defined. This micro-organism is a member of the genus Eubacterium but it is not closely related to Eubacterium limosum, the type species of the genus Eubacterium, and is nearer to Collinsella aerofaciens and Coriobacterium glomerans. A PCR-based identification system using species-specific primers designed on the basis of DNA sequences encoding the 16S rRNA of strains of Eubacterium lentum, Collinsella aerofaciens and Coriobacterium glomerans is described. A species-specific primer set can distinguish Eubacterium lentum from Eubacterium limosum or closely related species including Collinsella aerofaciens, Coriobacterium glomerans and Atopobium species. This species-specific PCR method can be used to identify Eubacterium lentum-like species isolated from human faeces. On the basis of the 16S rRNA sequence divergence from Collinsella aerofaciens and Coriobacterium glomerans and the presence of unique phenotypic characters, a new genus, Eggerthella gen. nov., is proposed for Eubacterium lentum, with one species, Eggerthella lenta comb. nov. The type strain of Eggerthella lenta is JCM 9979T.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0020-7713
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
49 Pt 4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1725-32
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10555354-Bacterial Typing Techniques,
pubmed-meshheading:10555354-DNA, Bacterial,
pubmed-meshheading:10555354-DNA, Ribosomal,
pubmed-meshheading:10555354-DNA Primers,
pubmed-meshheading:10555354-Eubacterium,
pubmed-meshheading:10555354-Feces,
pubmed-meshheading:10555354-Genes, rRNA,
pubmed-meshheading:10555354-Humans,
pubmed-meshheading:10555354-Molecular Sequence Data,
pubmed-meshheading:10555354-Phylogeny,
pubmed-meshheading:10555354-Polymerase Chain Reaction,
pubmed-meshheading:10555354-RNA, Ribosomal, 16S,
pubmed-meshheading:10555354-Sequence Analysis, DNA,
pubmed-meshheading:10555354-Species Specificity
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pubmed:year |
1999
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pubmed:articleTitle |
Phylogenetic evidence for the transfer of Eubacterium lentum to the genus Eggerthella as Eggerthella lenta gen. nov., comb. nov.
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pubmed:affiliation |
Japan Collection of Microorganisms, Institute of Physical and Chemical Research (RIKEN), Saitama, Japan. kageyama@ulmus.riken.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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