Source:http://linkedlifedata.com/resource/pubmed/id/10553298
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1999-12-17
|
| pubmed:databankReference | |
| pubmed:abstractText |
Activated sludge communities which performed enhanced biological phosphate removal (EBPR) were phylogenetically analyzed by 16S rRNA-targeted molecular methods. Two anaerobic-aerobic sequencing batch reactors were operated with two different carbon sources (acetate vs. a complex mixture) for three years and showed anaerobic-aerobic cycles of polyhydroxybutyrate- (PHB) and phosphate-accumulation characteristic for EBPR-systems. In situ hybridization showed that the reactor fed with the acetate medium was dominated by bacteria phylogenetically related to the Rhodocyclus-group within the beta-Proteobacteria (81% of DAPI-stained cells). The reactor with the complex medium was also predominated by this phylogenetic group albeit at a lesser extent (23% of DAPI-stained cells). More detailed taxonomic information on the dominant bacteria in the acetate-reactor was obtained by constructing clone libraries of 16S rDNA fragments. Two different types of Rhodocyclus-like clones (R1 and R6) were retrieved. Type-specific in situ hybridization and direct rRNA-sequencing revealed that R6 was the type of the dominant bacteria. Staining of intracellular polyphosphate- and PHB-granules confirmed that the R6-type bacterium accumulates PHB and polyphosphate just as predicted by the metabolic models for EBPR. High similarities to 16S rDNA fragments from other EBPR-sludges suggest that R6-type organisms were present and may play an important role in EBPR in general. Although the R6-type bacterium is closely related to the genus Rhodocyclus, it did not grow phototrophically. Therefore, we propose a provisional new genus and species Candidatus Accumulibacter phosphatis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DAPI,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Hydroxybutyrates,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Sewage
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
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| pubmed:issn |
0723-2020
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
454-65
|
| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10553298-Aerobiosis,
pubmed-meshheading:10553298-Anaerobiosis,
pubmed-meshheading:10553298-Bacteria,
pubmed-meshheading:10553298-Base Sequence,
pubmed-meshheading:10553298-Biodegradation, Environmental,
pubmed-meshheading:10553298-DNA, Bacterial,
pubmed-meshheading:10553298-DNA, Ribosomal,
pubmed-meshheading:10553298-Hydroxybutyrates,
pubmed-meshheading:10553298-In Situ Hybridization,
pubmed-meshheading:10553298-Indoles,
pubmed-meshheading:10553298-Microscopy, Fluorescence,
pubmed-meshheading:10553298-Molecular Sequence Data,
pubmed-meshheading:10553298-Nucleic Acid Hybridization,
pubmed-meshheading:10553298-Phosphates,
pubmed-meshheading:10553298-Phylogeny,
pubmed-meshheading:10553298-RNA, Bacterial,
pubmed-meshheading:10553298-RNA, Ribosomal, 16S,
pubmed-meshheading:10553298-Sewage,
pubmed-meshheading:10553298-Staining and Labeling
|
| pubmed:year |
1999
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| pubmed:articleTitle |
Enrichment, phylogenetic analysis and detection of a bacterium that performs enhanced biological phosphate removal in activated sludge.
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| pubmed:affiliation |
Swiss Federal Institute for Environmental Science and Technology (EAWAG), Dübendorf, Switzerland.
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| pubmed:publicationType |
Journal Article
|