Source:http://linkedlifedata.com/resource/pubmed/id/10553118
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
1999-12-15
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| pubmed:abstractText |
The regional and cellular localisation of gamma-aminobutyric acid(A) (GABA(A)) receptors was investigated in the human basal ganglia using receptor autoradiography and immunohistochemical staining for five GABA(A) receptor subunits (alpha(1), alpha(2), alpha(3), beta(2, 3), and gamma(2)) and other neurochemical markers. The results demonstrated that GABA(A) receptors in the striatum showed considerable subunit heterogeneity in their regional distribution and cellular localisation. High densities of GABA(A) receptors in the striosome compartment contained the alpha(2), alpha(3), beta(2, 3), and gamma(2) subunits, and lower densities of receptors in the matrix compartment contained the alpha(1), alpha(2), alpha(3), beta(2,3), and gamma(2) subunits. Also, six different types of neurons were identified in the striatum on the basis of GABA(A) receptor subunit configuration, cellular and dendritic morphology, and chemical neuroanatomy. Three types of alpha(1) subunit immunoreactive neurons were identified: type 1, the most numerous (60%), were medium-sized aspiny neurons that were immunoreactive for parvalbumin and alpha(1), beta(2,3), and gamma(2) subunits; type 2 (38%) were medium-sized to large aspiny neurons immunoreactive for calretinin and alpha(1), alpha(3), beta(2,3), and gamma(2) subunits; and type 3 (2%) were large sparsely spiny neurons immunoreactive for alpha(1), alpha(3), beta(2,3), and gamma(2) subunits. Type 4 neurons were calbindin-positive and immunoreactive for alpha(2), alpha(3), beta(2,3), and gamma(2) subunits. The remaining neurons were immunoreactive for choline acetyltransferase (ChAT) and alpha(3) subunit (type 5) or were neuropeptide Y-positive with no GABA(A) receptor subunit immunoreactivity (type 6). The globus pallidus contained three types of neurons: types 1 and 2 were large neurons and were immunoreactive for alpha(1), alpha(3), beta(2,3), and gamma(2) subunits and for parvalbumin alone (type 1) or for both parvalbumin and calretinin (type 2); type 3 neurons were medium-sized and immunoreactive for calretinin and alpha(1), beta(2, 3), and gamma(2) subunits. These results show that the subunit composition of GABA(A) receptors displays considerable regional and cellular variation in the human striatum but are more homogeneous in the globus pallidus.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0021-9967
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:day |
20
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| pubmed:volume |
415
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
313-40
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10553118-Acetylcholinesterase,
pubmed-meshheading:10553118-Adult,
pubmed-meshheading:10553118-Aged,
pubmed-meshheading:10553118-Aged, 80 and over,
pubmed-meshheading:10553118-Animals,
pubmed-meshheading:10553118-Autoradiography,
pubmed-meshheading:10553118-Basal Ganglia,
pubmed-meshheading:10553118-Blotting, Western,
pubmed-meshheading:10553118-Cell Size,
pubmed-meshheading:10553118-Female,
pubmed-meshheading:10553118-Fluorescent Antibody Technique,
pubmed-meshheading:10553118-Globus Pallidus,
pubmed-meshheading:10553118-Humans,
pubmed-meshheading:10553118-Immunohistochemistry,
pubmed-meshheading:10553118-Male,
pubmed-meshheading:10553118-Microscopy, Confocal,
pubmed-meshheading:10553118-Middle Aged,
pubmed-meshheading:10553118-Neostriatum,
pubmed-meshheading:10553118-Neurons,
pubmed-meshheading:10553118-Receptors, GABA-A
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| pubmed:year |
1999
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| pubmed:articleTitle |
Regional and cellular localisation of GABA(A) receptor subunits in the human basal ganglia: An autoradiographic and immunohistochemical study.
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| pubmed:affiliation |
Department of Anatomy with Radiology, Faculty of Medicine and Health Science, University of Auckland, Auckland, New Zealand. h.waldvogel@auckland.ac.nz
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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