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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-11-10
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pubmed:abstractText |
Growing evidence suggests that phosphoinositides play an important role in membrane traffic. A polyphosphoinositide phosphatase, synaptojanin 1, was identified as a major presynaptic protein associated with endocytic coated intermediates. We report here that synaptojanin 1-deficient mice exhibit neurological defects and die shortly after birth. In neurons of mutant animals, PI(4,5)P2 levels are increased, and clathrin-coated vesicles accumulate in the cytomatrix-rich area that surrounds the synaptic vesicle cluster in nerve endings. In cell-free assays, reduced phosphoinositide phosphatase activity correlated with increased association of clathrin coats with liposomes. Intracellular recording in hippocampal slices revealed enhanced synaptic depression during prolonged high-frequency stimulation followed by delayed recovery. These results provide genetic evidence for a crucial role of phosphoinositide metabolism in synaptic vesicle recycling.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0092-8674
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pubmed:author |
pubmed-author:CremonaOO,
pubmed-author:DanielaCC,
pubmed-author:De CamilliPP,
pubmed-author:Di PaoloGG,
pubmed-author:FlavellR ARA,
pubmed-author:KimW TWT,
pubmed-author:LüthiAA,
pubmed-author:McCormickD ADA,
pubmed-author:NemotaKK,
pubmed-author:ShearsS BSB,
pubmed-author:TakeiKK,
pubmed-author:WestO DODJr
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
179-88
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10535736-Animals,
pubmed-meshheading:10535736-Cell-Free System,
pubmed-meshheading:10535736-Cerebral Cortex,
pubmed-meshheading:10535736-Coated Pits, Cell-Membrane,
pubmed-meshheading:10535736-Endocytosis,
pubmed-meshheading:10535736-Enzyme Inhibitors,
pubmed-meshheading:10535736-Exons,
pubmed-meshheading:10535736-Hippocampus,
pubmed-meshheading:10535736-Membrane Potentials,
pubmed-meshheading:10535736-Mice,
pubmed-meshheading:10535736-Mice, Knockout,
pubmed-meshheading:10535736-Microscopy, Electron,
pubmed-meshheading:10535736-Nerve Endings,
pubmed-meshheading:10535736-Nerve Tissue Proteins,
pubmed-meshheading:10535736-Neurons,
pubmed-meshheading:10535736-Phosphatidylinositols,
pubmed-meshheading:10535736-Phosphoric Monoester Hydrolases,
pubmed-meshheading:10535736-Synaptic Vesicles
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pubmed:year |
1999
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pubmed:articleTitle |
Essential role of phosphoinositide metabolism in synaptic vesicle recycling.
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pubmed:affiliation |
Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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