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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-12-7
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pubmed:databankReference |
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pubmed:abstractText |
p21(Cip1/Waf1) inhibits cell-cycle progression by binding to G1 cyclin/CDK complexes and proliferating cell nuclear antigen (PCNA) through its N- and C-terminal domains, respectively. Here, we report a novel p21(Cip1/Waf1)-interacting protein, Ciz1 (for Cip1 interacting zinc finger protein), which contains polyglutamine repeats and glutamine-rich region in the N-terminus as well as three zinc-finger motifs and one MH3 (matrin 3-homologous domain 3) in the C-terminal region. Ciz1 bound to the N-terminal, the CDK2-interacting part of p21(Cip1/Waf1), and the interaction was disrupted by the overexpression of CDK2. A region of about 150 amino acids containing the first zinc-finger motif in Ciz1 was the binding site for p21(Cip1/Waf1). When Ciz1 and p21(Cip1/Waf1) were individually overexpressed in U2-OS cells, they mostly localized in the nucleus. However, coexpression of Ciz1 induced cytoplasmic distribution of p21(Cip1/Waf1). These data indicate that Ciz1 is a unique nuclear protein that regulates the cellular localization of p21(Cip1/Waf1).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/CDK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
264
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
457-64
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10529385-Amino Acid Sequence,
pubmed-meshheading:10529385-Binding Sites,
pubmed-meshheading:10529385-CDC2-CDC28 Kinases,
pubmed-meshheading:10529385-Cell Line,
pubmed-meshheading:10529385-Cell Nucleus,
pubmed-meshheading:10529385-Cloning, Molecular,
pubmed-meshheading:10529385-Cyclin-Dependent Kinase 2,
pubmed-meshheading:10529385-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:10529385-Cyclin-Dependent Kinases,
pubmed-meshheading:10529385-Cyclins,
pubmed-meshheading:10529385-DNA, Complementary,
pubmed-meshheading:10529385-Enzyme Inhibitors,
pubmed-meshheading:10529385-Gene Library,
pubmed-meshheading:10529385-Humans,
pubmed-meshheading:10529385-Molecular Sequence Data,
pubmed-meshheading:10529385-Mutation,
pubmed-meshheading:10529385-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10529385-RNA, Messenger,
pubmed-meshheading:10529385-Zinc Fingers
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pubmed:year |
1999
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pubmed:articleTitle |
Cloning and characterization of a novel p21(Cip1/Waf1)-interacting zinc finger protein, ciz1.
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pubmed:affiliation |
Institute of Life Science, Kurume University, Aikawa-machi 2432-3, Kurume, 839-0861, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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