Linked Life Data

10515215

Source:http://linkedlifedata.com/resource/pubmed/id/10515215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-11-3
pubmed:abstractText
The domestic dog (Canis familiaris) is widely used as a model in the study of human disease. However, many of the 78 chromosomes comprising the canine karyotype are extremely difficult to identify reliably by classical cytogenetics. This has been a major hindrance to molecular cytogenetic studies of this species. The Animal Health Trust and the Sanger Centre have developed a set of canine whole chromosome-specific fluorescence in situ hybridisation (FISH) probes (chromosome paints). We have used these chromosome paints to identify unequivocally each chromosome in a metaphase spread. An increasing number of laboratories are making use of cooled CCD cameras and sophisticated software for FISH mapping. Consequently, there is a major trend towards the use of DAPI banding for concurrent chromosome identification during FISH analyses in a range of species. Here we present, for the first time, a complete DAPI banded karyotype of the dog in which each chromosome has been accurately placed, together with a 460-band DAPI ideogram. These data will facilitate the accurate assignment of FISH-mapped loci to all chromosomes comprising the karyotype and form the basis for an agreed standard of the dog karyotype.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0967-3849
pubmed:author
pubmed:issnType
Print
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-6
pubmed:dateRevised
2009-9-29
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome-specific paint probes.
pubmed:affiliation
Animal Health Trust, Kentford, Suffolk, UK. mtbreen@hgmp.mrc.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't