rdf:type |
|
lifeskim:mentions |
umls-concept:C0010260,
umls-concept:C0017262,
umls-concept:C0019932,
umls-concept:C0024687,
umls-concept:C0027895,
umls-concept:C0035150,
umls-concept:C0185117,
umls-concept:C0332185,
umls-concept:C0946597,
umls-concept:C1160191,
umls-concept:C1314939,
umls-concept:C1521991,
umls-concept:C1880022,
umls-concept:C2911684
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pubmed:dateCreated |
1999-12-21
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pubmed:databankReference |
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pubmed:abstractText |
Methyl farnesoate, the crustacean juvenoid, is synthesized and secreted from the mandibular organs of crustaceans under the negative control of the sinus gland-derived mandibular organ-inhibiting hormone (MO-IH). Previously we isolated and sequenced two isoforms, MO-IH-1 and MO-IH-2, differing by just one amino acid, from sinus glands of the edible crab, Cancer pagurus. We now report the isolation of cDNAs encoding MO-IH-1 and MO-IH-2 by a combination of reverse-transcriptase-mediated PCR in conjunction with 5' and 3' rapid amplification of cDNA ends ('RACE'). Full-length clones of MO-IH-1 and MO-IH-2 encoded a 34-residue putative signal peptide and the mature 78-residue MO-IH sequences. Northern blot analysis of various tissues showed that MO-IH expression is confined to the X-organ (a cluster of perikarya within the eye). Southern blot analysis indicated that there are approx. 10 copies of the gene for MO-IH in C. pagurus. Additional Southern blotting experiments detected MO-IH-hybridizing bands in another Cancer species, C. antennarius. In support of this, an HPLC-radioimmunoassay analysis of sinus gland extracts of C. antennarius and C. magister also revealed MO-IH-like immunoreactivity.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-1601108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-1601279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-17778635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-2169734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-7537497,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-7685466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-7957869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-822353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-8224217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-8224920,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-8335230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-8424625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-8590372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-8662685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-9299429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-9450390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-9548218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-9701616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-9729474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-9731186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10510300-9931416
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
343 Pt 2
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pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
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pubmed:pagination |
355-60
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:10510300-Amino Acid Sequence,
pubmed-meshheading:10510300-Animals,
pubmed-meshheading:10510300-Base Sequence,
pubmed-meshheading:10510300-Blotting, Southern,
pubmed-meshheading:10510300-Brachyura,
pubmed-meshheading:10510300-Cloning, Molecular,
pubmed-meshheading:10510300-Databases, Factual,
pubmed-meshheading:10510300-Female,
pubmed-meshheading:10510300-Gene Dosage,
pubmed-meshheading:10510300-Insect Hormones,
pubmed-meshheading:10510300-Molecular Sequence Data,
pubmed-meshheading:10510300-Neuropeptides,
pubmed-meshheading:10510300-Organ Specificity,
pubmed-meshheading:10510300-RNA, Messenger,
pubmed-meshheading:10510300-Radioimmunoassay,
pubmed-meshheading:10510300-Reproduction,
pubmed-meshheading:10510300-Sequence Alignment,
pubmed-meshheading:10510300-Sequence Homology, Amino Acid
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pubmed:year |
1999
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pubmed:articleTitle |
Molecular characterization and expression of mandibular organ-inhibiting hormone, a recently discovered neuropeptide involved in the regulation of growth and reproduction in the crab Cancer pagurus.
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pubmed:affiliation |
School of Biological Sciences, University of Liverpool, Life Sciences Building, Crown Street, Liverpool L69 7ZB, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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