Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-11-22
pubmed:abstractText
Activin-A is a member of the transforming growth factor-beta (TGF-beta) superfamily and is expressed by osteoblasts. However, the role of activin-A on osteoblasts is not clearly understood. We examined the effects of activin-A on osteoblast proliferation or differentiation, and mineralization by the osteoblasts in the first subcultures of fetal rat osteoblasts obtained from calvarial bones. Exogenous activin-A led to impaired formation of bone nodules in a dose-dependent manner, although it did not influence cell proliferation using an MTT assay. This inhibitory effect depended upon the time at which activin-A was added to the culture media, and the effect was most significant when addition took place at the early phase of the culture. In addition, exogenous activin-A inhibited gene expression of type I procollagen, alkaline phosphatase, osteonectin, and osteopontin in the cultured cells using Northern blot analysis. The peak of osteocalcin mRNA was delayed. Gene expression for TGF-beta was not influenced by exogenous activin-A. The betaA subunit (activin-A) mRNA was detected during the early phase of this culture. These results indicate that activin-A inhibited early differentiation of the fetal rat calvarial cells, or osteoblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
206-14
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10502293-Activins, pubmed-meshheading:10502293-Alkaline Phosphatase, pubmed-meshheading:10502293-Animals, pubmed-meshheading:10502293-Bone and Bones, pubmed-meshheading:10502293-Cell Differentiation, pubmed-meshheading:10502293-Cells, Cultured, pubmed-meshheading:10502293-Dose-Response Relationship, Drug, pubmed-meshheading:10502293-Inhibins, pubmed-meshheading:10502293-Osteoblasts, pubmed-meshheading:10502293-Osteocalcin, pubmed-meshheading:10502293-Osteonectin, pubmed-meshheading:10502293-Osteopontin, pubmed-meshheading:10502293-Procollagen, pubmed-meshheading:10502293-RNA, Messenger, pubmed-meshheading:10502293-Rats, pubmed-meshheading:10502293-Rats, Sprague-Dawley, pubmed-meshheading:10502293-Sialoglycoproteins, pubmed-meshheading:10502293-Skull, pubmed-meshheading:10502293-Time Factors
pubmed:year
1999
pubmed:articleTitle
Inhibitory effects of activin-A on osteoblast differentiation during cultures of fetal rat calvarial cells.
pubmed:affiliation
Department of Orthopaedic Surgery, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't