Linked Life Data

10501828

Source:http://linkedlifedata.com/resource/pubmed/id/10501828

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-11-2
pubmed:abstractText
The role of glycosylation on voltage-dependent channel gating for the cloned human cardiac sodium channel (hH1a) and the adult rat skeletal muscle isoform (microl) was investigated in HEK293 cells transiently transfected with either hH1a or microl cDNA. The contribution of sugar residues to channel gating was examined in transfected cells pretreated with various glycosidase and enzyme inhibitors to deglycosylate channel proteins. Pretreating transfected cells with enzyme inhibitors castanospermine and swainsonine, or exo-glycosidase neuroaminidase caused 7 to 9 mV depolarizing shifts of V(1/2) for steady-state activation of hH1a, while deglycosylation with corresponding drugs elicited about the same amount of depolarizing shifts (8 to 9 mV) of V(1/2) for steady-state activation of microl. Elevated concentrations of extracellular Mg(2+) significantly masked the castanospermine-elicited depolarizing shifts of V(1/2) for steady-state activation in both transfected hH1a and microl. For steady-state activation, deglycosylation induced depolarizing shifts of V(1/2) for hH1a (10.6 to 12 mV), but hyperpolarizing shifts for microl (3.6 to 4.4 mV). Pretreatment with neuraminidase had no significant effects on single-channel conductance, the mean open time, and the open probability. These data suggest that glycosylation differentially regulates Na channel function in heart and skeletal muscle myocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-2631
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
171
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-207
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Glycosylation influences voltage-dependent gating of cardiac and skeletal muscle sodium channels.
pubmed:affiliation
Department of Physiology, University of Kentucky College of Medicine, Lexington, KY 40536-0298, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't