Linked Life Data

10498396

Source:http://linkedlifedata.com/resource/pubmed/id/10498396

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1999-11-10
pubmed:abstractText
The folate receptor (FR) type alpha is known to be frequently overexpressed in ovarian cancer and is the target for a number of novel experimental cancer therapies. The relative levels of FR expression among specific cell types and its relationship to malignant transformation have not been adequately established because of several inherent limitations of the immunocytochemical approaches used previously. We used a quantitative in situ hybridization method to examine the expression of the mRNAs for the known isoforms of FR in paraffin-embedded tissue sections of multiple samples of the various subtypes of ovarian, uterine, and cervical cancers. Benign lesions, as well as the various normal cell types in the ovary, the uterus, and the cervix, were examined similarly. FR mRNA levels were quantitated relative to the transcript levels for beta-actin using NIH Image 1.57 computer software. The results show that the ovary, the uterus, and the cervix present different patterns of FR regulation in differentiation and in malignancy. In the ovary, benign differentiation of the germinal epithelium into mucinous or serous tumors or malignant transformation into mucinous tumors is associated with down-regulation of FR-alpha, whereas FR-alpha expression is retained in malignant lesions of serous and endometrioid differentiation. In contrast, malignant transformation of the glandular epithelial cells of the uterine endometrium is associated with de novo expression of FR-alpha. Heterogeneity in FR expression within malignant ovarian and uterine tumors is related to differentiation. In contrast to the uterus, malignant transformation of glandular epithelial cells in the cervix may frequently result in down-regulation of FR-alpha. These results shed new light for the identification of malignancies suitable for FR-mediated therapies and for prognostic/diagnostic applications of FR. They also provide a phenomenological basis for molecular studies of FR regulation in malignant cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1055-9965
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
775-82
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10498396-Carrier Proteins, pubmed-meshheading:10498396-Cell Transformation, Neoplastic, pubmed-meshheading:10498396-Cervix Uteri, pubmed-meshheading:10498396-Down-Regulation, pubmed-meshheading:10498396-Female, pubmed-meshheading:10498396-Folate Receptors, GPI-Anchored, pubmed-meshheading:10498396-Folic Acid, pubmed-meshheading:10498396-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10498396-Genital Neoplasms, Female, pubmed-meshheading:10498396-Humans, pubmed-meshheading:10498396-In Situ Hybridization, pubmed-meshheading:10498396-Ovarian Neoplasms, pubmed-meshheading:10498396-Ovary, pubmed-meshheading:10498396-RNA, Messenger, pubmed-meshheading:10498396-RNA Probes, pubmed-meshheading:10498396-Receptors, Cell Surface, pubmed-meshheading:10498396-Sensitivity and Specificity, pubmed-meshheading:10498396-Uterine Cervical Neoplasms, pubmed-meshheading:10498396-Uterine Neoplasms, pubmed-meshheading:10498396-Uterus
pubmed:year
1999
pubmed:articleTitle
Expression of folate receptor type alpha in relation to cell type, malignancy, and differentiation in ovary, uterus, and cervix.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Medical College of Ohio, Toledo 43614-5804, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.