Linked Life Data

10490610

Source:http://linkedlifedata.com/resource/pubmed/id/10490610

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2000-2-3
pubmed:abstractText
mRNAs are monitored for errors in gene expression by RNA surveillance, in which mRNAs that cannot be fully translated are degraded by the nonsense-mediated mRNA decay pathway (NMD). RNA surveillance ensures that potentially deleterious truncated proteins are seldom made. NMD pathways that promote surveillance have been found in a wide range of eukaryotes. In Saccharomyces cerevisiae, the proteins encoded by the UPF1, UPF2, and UPF3 genes catalyze steps in NMD and are required for RNA surveillance. In this report, we show that the Upf proteins are also required to control the total accumulation of a large number of mRNAs in addition to their role in RNA surveillance. High-density oligonucleotide arrays were used to monitor global changes in the yeast transcriptome caused by loss of UPF gene function. Null mutations in the UPF genes caused altered accumulation of hundreds of mRNAs. The majority were increased in abundance, but some were decreased. The same mRNAs were affected regardless of which of the three UPF gene was inactivated. The proteins encoded by UPF-dependent mRNAs were broadly distributed by function but were underrepresented in two MIPS (Munich Information Center for Protein Sequences) categories: protein synthesis and protein destination. In a UPF(+) strain, the average level of expression of UPF-dependent mRNAs was threefold lower than the average level of expression of all mRNAs in the transcriptome, suggesting that highly abundant mRNAs were underrepresented. We suggest a model for how the abundance of hundreds of mRNAs might be controlled by the Upf proteins.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-10098411, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-1569946, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-1748286, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-2038328, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-2204810, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-388431, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-3900423, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-6300772, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-7050668, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-7479783, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-7489507, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-7489520, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-7883167, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-7883168, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-8104846, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-8346213, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-8595562, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-8811857, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-8855285, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9064659, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9268361, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9415887, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9570320, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9634850, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9644970, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9742129, http://linkedlifedata.com/resource/pubmed/commentcorrection/10490610-9799255
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6710-9
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Yeast Upf proteins required for RNA surveillance affect global expression of the yeast transcriptome.
pubmed:affiliation
Laboratories of Genetics and Molecular Biology, University of Wisconsin, Madison, Wisconsin 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't