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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1999-9-23
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pubmed:abstractText |
We have shown that Werner syndrome (WRN) fibroblast cell lines are unusually sensitive to the DNA-damaging agent 4-nitroquinoline 1-oxide (4NQO), though not to gamma radiation or to hydrogen peroxide. The fusion of 4NQO-sensitive WRN and 4NQO-resistant control fibroblast cell lines generated proliferating WRN x control cell hybrids that expressed WRN protein and were 4NQO-resistant. These results establish the recessive nature of 4NQO sensitivity in WRN cell lines and provide a cellular assay for WRN protein function.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0340-6717
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
105
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
132-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10480367-4-Nitroquinoline-1-oxide,
pubmed-meshheading:10480367-Alleles,
pubmed-meshheading:10480367-Blotting, Western,
pubmed-meshheading:10480367-Cell Fusion,
pubmed-meshheading:10480367-Cell Line, Transformed,
pubmed-meshheading:10480367-Cell Survival,
pubmed-meshheading:10480367-DNA Damage,
pubmed-meshheading:10480367-Dose-Response Relationship, Drug,
pubmed-meshheading:10480367-Dose-Response Relationship, Radiation,
pubmed-meshheading:10480367-Drug Resistance,
pubmed-meshheading:10480367-Fibroblasts,
pubmed-meshheading:10480367-Genotype,
pubmed-meshheading:10480367-Humans,
pubmed-meshheading:10480367-Mutagens,
pubmed-meshheading:10480367-Phenotype,
pubmed-meshheading:10480367-Transfection,
pubmed-meshheading:10480367-Werner Syndrome
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pubmed:articleTitle |
Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines.
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pubmed:affiliation |
University of Washington, Department of Pathology, Seattle 98195-7705, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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