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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1999-10-21
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pubmed:abstractText |
Apo-2L is a new member of the tumour necrosis factor (TNF) family shown to induce apoptosis in a number of tumour cell lines. Apo-2L mRNA is expressed by numerous human tissues. Here we report that Apo-2L is expressed and utilized by human Natural Killer (NK) cells. NK cells were shown to express surface Apo-2L in response to interleukin 2 (IL-2) activation, and this response was restricted to the CD3(-)population of the NK cells. Apo-2L mRNA and intracellular Apo-2L were present in both CD3(-)and CD3(+)NK cells; however, increased expression in response to IL-2 was only observed in CD3(-)CD56(+)cells. Also, IL-2-activated NK cells were shown to utilize membrane-bound Apo-2L in mediating lysis of Jurkat cells. Furthermore, Apo-2L-induced apoptosis of Jurkat cells was more rapid than FasL-induced apoptosis, indicating an important and distinct role for Apo-2L in apoptotic cell destruction. In conclusion, we report that NK cells express Apo-2L and that IL-2 activated CD3(-)NK cells utilize the Apo-2L pathway in mediating target cell lysis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FASLG protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TNF-Related Apoptosis-Inducing...,
http://linkedlifedata.com/resource/pubmed/chemical/TNFSF10 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
1043-4666
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
664-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10479402-Antibodies, Monoclonal,
pubmed-meshheading:10479402-Apoptosis,
pubmed-meshheading:10479402-Apoptosis Regulatory Proteins,
pubmed-meshheading:10479402-Cytotoxicity, Immunologic,
pubmed-meshheading:10479402-Fas Ligand Protein,
pubmed-meshheading:10479402-Gene Expression Regulation,
pubmed-meshheading:10479402-Humans,
pubmed-meshheading:10479402-Immunophenotyping,
pubmed-meshheading:10479402-Interleukin-2,
pubmed-meshheading:10479402-Jurkat Cells,
pubmed-meshheading:10479402-Killer Cells, Natural,
pubmed-meshheading:10479402-Membrane Glycoproteins,
pubmed-meshheading:10479402-RNA, Messenger,
pubmed-meshheading:10479402-Recombinant Proteins,
pubmed-meshheading:10479402-TNF-Related Apoptosis-Inducing Ligand,
pubmed-meshheading:10479402-Tumor Necrosis Factor-alpha
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pubmed:year |
1999
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pubmed:articleTitle |
Regulation of APO-2 ligand/trail expression in NK cells-involvement in NK cell-mediated cytotoxicity.
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pubmed:affiliation |
Department of Cancer Research and Molecular Biology, Norwegian University of Science and Technology, Trondheim, Norway. Ann-Charlotte.Johnsen@medisin.ntnu.no
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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