| pubmed-article:10471470 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10471470 | lifeskim:mentions | umls-concept:C1956416 | lld:lifeskim |
| pubmed-article:10471470 | lifeskim:mentions | umls-concept:C0005528 | lld:lifeskim |
| pubmed-article:10471470 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:10471470 | lifeskim:mentions | umls-concept:C1517294 | lld:lifeskim |
| pubmed-article:10471470 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:10471470 | pubmed:dateCreated | 1999-11-2 | lld:pubmed |
| pubmed-article:10471470 | pubmed:abstractText | Mouse and rabbit preimplantation embryos with intact zona pellucida were incubated for 3 hr with DNA-carrying constructs containing insulin as an internalizable ligand: (insulin-polylysine)-DNA and (insulin-polylysine)-DNA-(streptavidin-polylysine)-(biotinylated adenovirus). Video-intensified microscopy demonstrated that the constructs penetrated the zona pellucida and accumulated in the blastomere perinuclear space. The percentage of blastocysts formed was about 70% after incubation of zygotes and two-cell embryos with the constructs. Foreign DNA was detected after 51 hr in 80% of rabbit embryos and after 96 hr in 73% of mouse embryos. Inclusion of various adenoviruses into the construct improved foreign DNA preservation in early embryos. Blot hybridization revealed genome-integrated foreign DNA in 12- and 15-day mouse embryos and in a newborn. Thus, the ligand-mediated mechanism can be employed for introducing foreign genetic material into early mammalian embryos; insulin provides for delivery inside the cell and to the nucleus, while adenoviruses ensure release from endosomes. | lld:pubmed |
| pubmed-article:10471470 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10471470 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10471470 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10471470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10471470 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10471470 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10471470 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:10471470 | pubmed:issn | 1040-452X | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:IvanovaM MMM | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:NaroditskyB... | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:SobolevA SAS | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:NikitinV AVA | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:SmirnovaO AOA | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:ErnstL KLK | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:LandaVV | lld:pubmed |
| pubmed-article:10471470 | pubmed:author | pubmed-author:RosenkranzA... | lld:pubmed |
| pubmed-article:10471470 | pubmed:copyrightInfo | Copyright 1999 Wiley-Liss, Inc. | lld:pubmed |
| pubmed-article:10471470 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10471470 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:10471470 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10471470 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10471470 | pubmed:pagination | 112-20 | lld:pubmed |
| pubmed-article:10471470 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:10471470 | pubmed:meshHeading | pubmed-meshheading:10471470... | lld:pubmed |
| pubmed-article:10471470 | pubmed:meshHeading | pubmed-meshheading:10471470... | lld:pubmed |
| pubmed-article:10471470 | pubmed:meshHeading | pubmed-meshheading:10471470... | lld:pubmed |
| pubmed-article:10471470 | pubmed:meshHeading | pubmed-meshheading:10471470... | lld:pubmed |
| pubmed-article:10471470 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10471470 | pubmed:articleTitle | Receptor-mediated transport of foreign DNA into preimplantation mammalian embryos. | lld:pubmed |
| pubmed-article:10471470 | pubmed:affiliation | Laboratory of Genetic Engineering and Molecular Diagnostics of Microorganisms, Moscow, Russia. | lld:pubmed |
| pubmed-article:10471470 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10471470 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
