Source:http://linkedlifedata.com/resource/pubmed/id/10471470
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-11-2
|
| pubmed:abstractText |
Mouse and rabbit preimplantation embryos with intact zona pellucida were incubated for 3 hr with DNA-carrying constructs containing insulin as an internalizable ligand: (insulin-polylysine)-DNA and (insulin-polylysine)-DNA-(streptavidin-polylysine)-(biotinylated adenovirus). Video-intensified microscopy demonstrated that the constructs penetrated the zona pellucida and accumulated in the blastomere perinuclear space. The percentage of blastocysts formed was about 70% after incubation of zygotes and two-cell embryos with the constructs. Foreign DNA was detected after 51 hr in 80% of rabbit embryos and after 96 hr in 73% of mouse embryos. Inclusion of various adenoviruses into the construct improved foreign DNA preservation in early embryos. Blot hybridization revealed genome-integrated foreign DNA in 12- and 15-day mouse embryos and in a newborn. Thus, the ligand-mediated mechanism can be employed for introducing foreign genetic material into early mammalian embryos; insulin provides for delivery inside the cell and to the nucleus, while adenoviruses ensure release from endosomes.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
1040-452X
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Wiley-Liss, Inc.
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| pubmed:issnType |
Print
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| pubmed:volume |
54
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
112-20
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10471470-Adenoviridae,
pubmed-meshheading:10471470-Animals,
pubmed-meshheading:10471470-Blotting, Southern,
pubmed-meshheading:10471470-Cells, Cultured,
pubmed-meshheading:10471470-DNA,
pubmed-meshheading:10471470-Embryo, Mammalian,
pubmed-meshheading:10471470-Embryonic Development,
pubmed-meshheading:10471470-Endocytosis,
pubmed-meshheading:10471470-Female,
pubmed-meshheading:10471470-Gene Transfer Techniques,
pubmed-meshheading:10471470-Insulin,
pubmed-meshheading:10471470-Mice,
pubmed-meshheading:10471470-Mice, Inbred BALB C,
pubmed-meshheading:10471470-Microscopy, Video,
pubmed-meshheading:10471470-Models, Genetic,
pubmed-meshheading:10471470-Plasmids,
pubmed-meshheading:10471470-Pregnancy,
pubmed-meshheading:10471470-Rabbits,
pubmed-meshheading:10471470-Transfection
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Receptor-mediated transport of foreign DNA into preimplantation mammalian embryos.
|
| pubmed:affiliation |
Laboratory of Genetic Engineering and Molecular Diagnostics of Microorganisms, Moscow, Russia.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|