Linked Life Data

10462471

Source:http://linkedlifedata.com/resource/pubmed/id/10462471

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-10-1
pubmed:abstractText
The luciferase system was used to assay basal promoter activity of the murine factor H gene in the fibroblast cell line L929 (L cells). Thirteen nested deletion constructs were tested, and a region between -811 and -344 was found to have enhancer activity in the context of a heterologous promoter. This fragment was subdivided further and each of the two resulting subfragments also had enhancer activity. These subfragments each were shifted in electrophoretic mobility shift assays and were able to cross-inhibit each other in binding to a nuclear factor. Sequence analysis of these subfragments revealed the presence of an octamer in each subfragment, and a synthetic oligomer containing this octamer sequence was able to block binding in the mobility shift assay. Thus, this octamer sequence appears to play a major role in the basal expression of the factor H gene in L cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
262
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
315-8
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Regulation of complement factor H expression in L cells.
pubmed:affiliation
Department of Molecular Genetics and Microbiology, University of New Mexico School of Medicine, Albuquerque, New Mexico, 87131-5276, USA. dvik@unm.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.