Linked Life Data

10451423

Source:http://linkedlifedata.com/resource/pubmed/id/10451423

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-9-23
pubmed:abstractText
In the teleost fish Chalcalburnus chalcoides (Cyprinidae) the influence of metabolic inhibitors, substrates, coenzymes, and oxygen concentrations on spermatozoal parameters during motility and during immotile incubation was studied, the respiration rate was characterized, representative metabolite levels were measured, and the results were compared with Oncorhynchus mykiss (Salmonidae). In Chalcalburnus chalcoides the sperm motility rate, the average path swimming velocity, the motility duration, and the viability of immotile semen were significantly reduced in the presence of inhibitors of respiration (potassium cyanide, 2.4-dinitrophenol, atractyloside). Anaerobic conditions (<1 mg O(2)/liter) and inhibition of the tricarboxylic acid cycle by malonate and >7.5 mmol/liter succinate had similar effects on the sperm motility parameters and on the viability of immotile spermatozoa. Pyruvate and coenzyme A (an acyl-group carrier during oxidative carboxylation of pyruvate) prolonged the duration of sperm motility and the viability of immotile incubated spermatozoa, and also increased the spermatozoal respiration rate. Glucose levels significantly decreased during motility and during immotile storage and, under anaerobic conditions, the levels of lactate increased indicating that pyruvate derived from glycolysis. The respiration rate and the glycolytic rate significantly increased during motility. Therefore oxidative phosphorylation, tricarboxylic acid cycle, and aerobic glycolysis are central energy-supplying pathways for spermatozoa of Chalcalburnus chalcoides. The stimulatory effect of pyruvate and coenzyme A indicated that glycolysis is a rate-controlling pathway. Similar results were obtained for Oncorhynchus mykiss with the only exception that the stimulatory effect of coenzyme A was more significant than the stimulatory effect of pyruvate. When the sperm motility-activating saline solutions were optimized in aspects of energy supply, ionic composition, and osmolality, about 50% of the motile spermatozoa swam progressively (>20 mm/sec) for about 3 min in Chalcalburnus chalcoides and in Oncorhynchus mykiss. About 20% swam progressively for >2 hr in Chalcalburnus chalcoides and for >30 min in Oncorhynchus mykiss. J. Exp. Zool. 284:454-465, 1999.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-104X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
454-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10451423-2,4-Dinitrophenol, pubmed-meshheading:10451423-Animals, pubmed-meshheading:10451423-Atractyloside, pubmed-meshheading:10451423-Cell Respiration, pubmed-meshheading:10451423-Cell Survival, pubmed-meshheading:10451423-Citric Acid Cycle, pubmed-meshheading:10451423-Coenzyme A, pubmed-meshheading:10451423-Cyprinidae, pubmed-meshheading:10451423-Energy Metabolism, pubmed-meshheading:10451423-Fatty Acids, pubmed-meshheading:10451423-Glycolysis, pubmed-meshheading:10451423-Male, pubmed-meshheading:10451423-Malonates, pubmed-meshheading:10451423-Oncorhynchus mykiss, pubmed-meshheading:10451423-Oxidation-Reduction, pubmed-meshheading:10451423-Oxygen, pubmed-meshheading:10451423-Potassium Cyanide, pubmed-meshheading:10451423-Pyruvic Acid, pubmed-meshheading:10451423-Sperm Motility, pubmed-meshheading:10451423-Spermatozoa, pubmed-meshheading:10451423-Succinic Acid, pubmed-meshheading:10451423-Uncoupling Agents
pubmed:year
1999
pubmed:articleTitle
Sperm metabolism of the telost fishes Chalcalburnus chalcoides and Oncorhynchus mykiss and its relation to motility and viability.
pubmed:affiliation
Institute for Zoology, University of Salzburg, A-5020 Salzburg, Austria. Franz.Lahnsteiner@sbg.ac.at
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't