Linked Life Data

10442641

Source:http://linkedlifedata.com/resource/pubmed/id/10442641

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
31
pubmed:dateCreated
1999-8-30
pubmed:abstractText
The X region of the HTLV-I genome contains four major open reading frames (ORFs), two of which, termed x-I and x-II, are of still undefined biological significance. By indirect immunofluorescence and dual labeling with marker proteins, we demonstrate that p13II, an 87-amino acid protein coded by the x-II ORF, is selectively targeted to mitochondria. Mutational analysis revealed that mitochondrial targeting of p13II is directed by an atypical 10-amino acid signal sequence that is not cleaved upon import and is able to target the Green Fluorescent Protein to mitochondria. Expression of p13II results in specific alterations of mitochondrial morphology and distribution from a typical string-like, dispersed network to round-shaped clusters, suggesting that p13II might interfere with processes relying on an intact mitochondrial architecture. Functional studies of mitochondria with the cationic fluorochrome tetramethylrhodamine revealed that a subpopulation of the cells with p13II-positive mitochondria show a disruption in the mitochondrial inner membrane potential (Apsi), an early event observed in cells committed to apoptosis. Taken together, these results suggest novel virus-cell interactions that might be important in HTLV-I replication and/or pathogenicity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4505-14
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10442641-Alternative Splicing, pubmed-meshheading:10442641-Amino Acid Sequence, pubmed-meshheading:10442641-DNA Mutational Analysis, pubmed-meshheading:10442641-Fluorescent Antibody Technique, Indirect, pubmed-meshheading:10442641-Genes, pX, pubmed-meshheading:10442641-Genome, Viral, pubmed-meshheading:10442641-HeLa Cells, pubmed-meshheading:10442641-Human T-lymphotropic virus 1, pubmed-meshheading:10442641-Humans, pubmed-meshheading:10442641-Mitochondria, pubmed-meshheading:10442641-Molecular Sequence Data, pubmed-meshheading:10442641-Open Reading Frames, pubmed-meshheading:10442641-RNA, Messenger, pubmed-meshheading:10442641-RNA, Viral, pubmed-meshheading:10442641-Recombinant Fusion Proteins, pubmed-meshheading:10442641-Retroviridae Proteins, pubmed-meshheading:10442641-Transfection
pubmed:year
1999
pubmed:articleTitle
Mitochondrial targeting of the p13II protein coded by the x-II ORF of human T-cell leukemia/lymphotropic virus type I (HTLV-I).
pubmed:affiliation
Department of Oncology and Surgical Sciences, University of Padova, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't