Linked Life Data

10437827

Source:http://linkedlifedata.com/resource/pubmed/id/10437827

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-8-16
pubmed:databankReference
pubmed:abstractText
The notion of debranching enzyme activity as a participant in starch synthesis is gaining acceptance. Inconsistent reports from mutant analyses implicate either isoamylase or pullulanase as a determinant in amylopectin formation and whether wild-type plants utilize one or the other, or both, of these debranching enzymes in starch synthesis is unclear. Recent results on the sul mutant in maize suggest that both forms of debranching enzymes might be involved in amylopectin formation. We wished to find out if isoamylase takes part in starch synthesis by comparing isoamylase gene activity under three conditions: (1) during starch accumulation in developing sink tissues; (2) during starch degradation in germinating seeds; (3) in ectopic expression after applying sucrose, a starch precursor. We isolated the gene for barley isoamylase, isol, and analysed its expression and regulation in germinating seeds, developing endosperm and vegetative tissues, and compared the isoamylase gene expression in sink tissues from three different species. Our results indicate that isoamylase gene activity is involved in starch synthesis in wild-type plants and is modulated by sucrose.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
431-43
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10437827-Amino Acid Sequence, pubmed-meshheading:10437827-Base Sequence, pubmed-meshheading:10437827-Cloning, Molecular, pubmed-meshheading:10437827-DNA, Complementary, pubmed-meshheading:10437827-DNA, Plant, pubmed-meshheading:10437827-DNA Primers, pubmed-meshheading:10437827-Gene Expression Regulation, Developmental, pubmed-meshheading:10437827-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10437827-Gene Expression Regulation, Plant, pubmed-meshheading:10437827-Genes, Plant, pubmed-meshheading:10437827-Hordeum, pubmed-meshheading:10437827-Isoamylase, pubmed-meshheading:10437827-Molecular Sequence Data, pubmed-meshheading:10437827-Sequence Homology, Amino Acid, pubmed-meshheading:10437827-Solanum tuberosum, pubmed-meshheading:10437827-Starch, pubmed-meshheading:10437827-Sucrose, pubmed-meshheading:10437827-Triticum
pubmed:year
1999
pubmed:articleTitle
Analyses of isoamylase gene activity in wild-type barley indicate its involvement in starch synthesis.
pubmed:affiliation
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, Sweden.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't