Linked Life Data

10421790

Source:http://linkedlifedata.com/resource/pubmed/id/10421790

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1999-9-24
pubmed:abstractText
At birth, virtually all peripheral CD8(+) T cells express the CD28 co-stimulatory molecule, but healthy human adults accumulate CD28(-)CD8(+) T cells that often express the CD57 marker. While these CD28(-) subpopulations are known to exert effector-type functions, the generation, maintenance and regulation of CD28(-) (CD57(+) or CD57(-)) subpopulations remain unresolved. Here, we compared the differentiation of CD8(+)CD28(bright)CD57(-) T cells purified from healthy adults or neonates and propagated in IL-2, alone or with IL-4. With IL-2 alone, CD8(+)CD28(bright)CD57(-) T cell cultures yielded a prevailing CD28(-) subpopulation. The few persisting CD28(dim) and the major CD28(-) cells were characterized by similar telomere shortening at the plateau phase of cell growth. Cultures from adults donors generated four final CD8(+) phenotypes: a major CD28(-)CD57(+), and three minor CD28(-)CD57(-), CD28(dim)CD57(-) and CD28(dim)CD57(dim). These four end-stage CD8(+) subpopulations displayed a fairly similar representation of TCR V(beta) genes. In cultures initiated with umbilical cord blood, virtually all the original CD8(+)CD28(bright) T cells lost expression of CD28, but none acquired CD57 with IL-2 alone. IL-4 impacted on the differentiation pathways of the CD8(+)CD28(bright)CD57(-) T cells: the addition of IL-4 led both the neonatal and the adult lymphocytes to keep their expression of CD28. Thus, CD8(+)CD28(bright)CD57(-) T cells can give rise to four end-stage subpopulations, the balance of which is controlled by both the cytokine environment, IL-4 in particular, and the proportions of naive and memory CD8(+)CD28(+) T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1327-36
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10421790-Adult, pubmed-meshheading:10421790-Animals, pubmed-meshheading:10421790-Antigens, CD28, pubmed-meshheading:10421790-Antigens, CD57, pubmed-meshheading:10421790-CD8-Positive T-Lymphocytes, pubmed-meshheading:10421790-Cell Differentiation, pubmed-meshheading:10421790-Cells, Cultured, pubmed-meshheading:10421790-Cytokines, pubmed-meshheading:10421790-Female, pubmed-meshheading:10421790-Fetal Blood, pubmed-meshheading:10421790-Genes, T-Cell Receptor beta, pubmed-meshheading:10421790-Humans, pubmed-meshheading:10421790-Immunologic Memory, pubmed-meshheading:10421790-Interleukin-2, pubmed-meshheading:10421790-Interleukin-4, pubmed-meshheading:10421790-Leukocytes, Mononuclear, pubmed-meshheading:10421790-Lymphocyte Activation, pubmed-meshheading:10421790-Mice, pubmed-meshheading:10421790-Middle Aged, pubmed-meshheading:10421790-Pregnancy, pubmed-meshheading:10421790-T-Lymphocyte Subsets, pubmed-meshheading:10421790-Telomere
pubmed:year
1999
pubmed:articleTitle
Peripheral human CD8(+)CD28(+)T lymphocytes give rise to CD28(-)progeny, but IL-4 prevents loss of CD28 expression.
pubmed:affiliation
Service d'Immunologie, EA 2686, Centre Hospitalier et Universitaire de Lille, 59045 Lille, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't