Linked Life Data

10419628

Source:http://linkedlifedata.com/resource/pubmed/id/10419628

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-9-8
pubmed:abstractText
CO2-capture methods have been used for assaying many decarboxylating enzymes including hydroxylation-coupled decarboxylation reactions. The traditional CO2-capture method involves performing the reaction in capped tubes and radiometric measurement of trapped 14CO2 by scintillation counting. In this report, a 14CO2-capture method in a 96-well microtiter plate format has been developed and a phosphor imaging system has been employed for sample measurement. The new assay method has been used successfully to assay aspartyl-beta-hydroxylase activity in microtiter plate format. The results obtained here compare favorably with those obtained from the traditional tube method. The method is sensitive, suitable for high throughput, and generally applicable to many CO2-releasing enzyme assays.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-2697
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
137-42
pubmed:dateRevised
2002-11-1
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Development of a carbon dioxide-capture assay in microtiter plate for aspartyl-beta-hydroxylase.
pubmed:affiliation
DuPont Pharmaceuticals Research Laboratories, DuPont Pharmaceuticals Company, Wilmington, Delaware 19880-0400, USA.
pubmed:publicationType
Journal Article