Source:http://linkedlifedata.com/resource/pubmed/id/10417761
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1999-8-13
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pubmed:abstractText |
Expression of prostaglandin-H-synthase (PGHS) isozymes was analyzed in 50 biopsies of normal human skin and of pre-malignant and malignant skin lesions, by means of quantitative RT-PCR, immunoprecipitation and Western blotting, as well as immunohistochemistry. Normal skin constitutively expressed PGHS-1 in all cell layers of the epidermis, in endothelial cells of small blood vessels and in sweat-gland epithelium. PGHS-2 expression was very low and restricted to a few keratinocytes of the interfollicular and follicular epidermis. Steady-state concentrations of PGHS-1 and PGHS-2 mRNA were similar in normal skin and in basal-cell carcinomas, but PGHS-1 mRNA was reduced and PGHS-2 mRNA was elevated in actinic keratoses, squamous-cell carcinomas and keratoacanthomas. PGHS-1 protein was detected in all tumor biopsies, being occasionally increased in basal-cell carcinomas. High amounts of PGHS-2 protein were found in actinic keratoses, squamous-cell carcinomas and keratoacanthomas, but not in basal-cell carcinomas. Four malignant melanomas included in this study contained PGHS-1 but no PGHS-2 protein. Immunohistochemical analysis of the biopsies identified keratinocytes, in addition to cells of inflammatory infiltrates and of dendritic morphology, as the major PGHS-expressing cell types. PGHS-2-specific signals were spread throughout the epidermal part of actinic keratoses and squamous-cell carcinomas. These data suggest that constitutive up-regulation of PGHS-2 expression is a consistent pre-malignant event in squamous-cell cancer development in man, as it is in animal models of skin carcinogenesis. Thus, pre-cancerous lesions such as actinic keratoses present a likely target for chemoprevention of skin cancer by selective PGHS-2 inhibitors.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0020-7136
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1999 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
648-56
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pubmed:dateRevised |
2007-7-24
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pubmed:meshHeading |
pubmed-meshheading:10417761-Aged,
pubmed-meshheading:10417761-Anti-Inflammatory Agents, Non-Steroidal,
pubmed-meshheading:10417761-Cyclooxygenase 1,
pubmed-meshheading:10417761-Cyclooxygenase 2,
pubmed-meshheading:10417761-Humans,
pubmed-meshheading:10417761-Isoenzymes,
pubmed-meshheading:10417761-Male,
pubmed-meshheading:10417761-Membrane Proteins,
pubmed-meshheading:10417761-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:10417761-RNA, Messenger,
pubmed-meshheading:10417761-RNA, Neoplasm,
pubmed-meshheading:10417761-Skin,
pubmed-meshheading:10417761-Skin Neoplasms,
pubmed-meshheading:10417761-Up-Regulation
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pubmed:year |
1999
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pubmed:articleTitle |
Prostaglandin-H-synthase isozyme expression in normal and neoplastic human skin.
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pubmed:affiliation |
Research Program, Tumor Cell Regulation, Deutsches Krebsforschungszentrum, Heidelberg, Germany. K.Mueller-Decker@DKFZ-Heidelberg.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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