Linked Life Data

10417261

Source:http://linkedlifedata.com/resource/pubmed/id/10417261

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-9-13
pubmed:abstractText
Forty-two antigenic domains were identified across the hepatitis A virus (HAV) polyprotein by using a set of 237 overlapping 20-mer synthetic peptides spanning the entire HAV polyprotein and a panel of serum samples from acutely HAV-infected patients. The term "antigenic domain" is used in this study to define a protein region spanned with consecutive overlapping immunoreactive peptides. Nineteen antigenic domains were found within the structural proteins, and 22 were found within the nonstructural proteins, with 1 domain spanning the junction of VP1 and P2A proteins. Five of these domains were considered immunodominant, as judged by both the breadth and the strength of their immunoreactivity. One domain is located within the VP2 protein at position 57-90 aa. A second domain, located at position 767-842 aa, contains the C-terminal part of the VP1 protein and the entire P2A protein. A third domain, located at position 1403-1456 aa, comprises the C-terminal part of the P2C protein and the N-terminal half of the P3A protein. The fourth domain, located at position 1500-1519 aa, includes almost the entire P3B, and the last domain, located at position 1719-1764 aa, contains the C-terminal region of the P3C protein and the N-terminal region of the P3D protein. It is interesting to note that four of the five most immunoreactive domains are derived from small HAV proteins and/or encompass protein cleavage sites separating different HAV proteins. The HAV-specific immunoreactivity of each antigenically reactive peptide was confirmed by using seven HAV seroconversion panels. Collectively, these data demonstrate that HAV structural and nonstructural proteins contain antigenic epitopes that can be efficiently modeled with short synthetic peptides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
260
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
260-72
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed-meshheading:10417261-Amino Acid Sequence, pubmed-meshheading:10417261-Animals, pubmed-meshheading:10417261-Antibodies, Viral, pubmed-meshheading:10417261-Antigens, Viral, pubmed-meshheading:10417261-Epitope Mapping, pubmed-meshheading:10417261-Epitopes, pubmed-meshheading:10417261-Hepatitis A, pubmed-meshheading:10417261-Hepatovirus, pubmed-meshheading:10417261-Humans, pubmed-meshheading:10417261-Immune Sera, pubmed-meshheading:10417261-Immunodominant Epitopes, pubmed-meshheading:10417261-Immunoglobulin G, pubmed-meshheading:10417261-Immunoglobulin M, pubmed-meshheading:10417261-Molecular Sequence Data, pubmed-meshheading:10417261-Pan troglodytes, pubmed-meshheading:10417261-Peptide Fragments, pubmed-meshheading:10417261-Viral Nonstructural Proteins, pubmed-meshheading:10417261-Viral Proteins, pubmed-meshheading:10417261-Viral Structural Proteins
pubmed:year
1999
pubmed:articleTitle
Antigenic epitopes of the hepatitis A virus polyprotein.
pubmed:affiliation
Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia, 30333, USA. yek0@cdc.gov
pubmed:publicationType
Journal Article