Linked Life Data

10410956

Source:http://linkedlifedata.com/resource/pubmed/id/10410956

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1999-9-14
pubmed:abstractText
A radiochemical assay was developed to measure the activity of dihydropyrimidinase (DHP) in human liver homogenates. The method is based on the separation of radiolabeled dihydrouracil from N-carbamyl-beta-alanine by HPLC with on-line detection of radioactivity combined with detection of 14CO2 by liquid scintillation counting. The assay was linear with time and protein concentration. The minimum amount of radiolabeled products which could be determined proved to be 12 pmol using a purified stock solution of [2-(14)C]-5,6-dihydrouracil. This highly sensitive assay is especially suitable to identify patients with a dihydropyrimidinase deficiency.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1387-2273
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
729
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
307-14
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Radiochemical assay for determination of dihydropyrimidinase activity using reversed-phase high-performance liquid chromatography.
pubmed:affiliation
Academic Medical Center, Laboratory Genetic Metabolic Diseases, Amsterdam, The Netherlands.
pubmed:publicationType
Journal Article