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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-8-9
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pubmed:abstractText |
The mi locus encodes the mi transcription factor (MITF), a member of the basic-helix-loop-helix-leucine zipper protein family of transcription factors. MITF binds the alphaB1/AML1 subtype of the alpha subunit of the polyomavirus enhancer binding protein 2 (PEBP2). These two transcription factors synergistically transactivate the mouse mast cell protease 6 (MMCP-6) gene. The interaction of PEBP2 with MITF is mediated through the region carboxy-terminal to the DNA-binding Runt domain. In the present study, we examined the region of MITF that is responsible for the interaction with PEBP2. The MITF mutant that lacked the region aa 67-152 did not bind PEBP2, and the mutant that lacked the region aa 1-152 lost the synergistic function in the transactivation of the MMCP-6 promoter. We conclude that the region amino-terminal to the basic region of MITF is required for physical and functional interactions with PEBP2.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
261
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10405322-Animals,
pubmed-meshheading:10405322-DNA-Binding Proteins,
pubmed-meshheading:10405322-Dimerization,
pubmed-meshheading:10405322-Humans,
pubmed-meshheading:10405322-Jurkat Cells,
pubmed-meshheading:10405322-Mice,
pubmed-meshheading:10405322-Microphthalmia-Associated Transcription Factor,
pubmed-meshheading:10405322-Protein Binding,
pubmed-meshheading:10405322-Protein Isoforms,
pubmed-meshheading:10405322-Recombinant Fusion Proteins,
pubmed-meshheading:10405322-Sequence Deletion,
pubmed-meshheading:10405322-Transcription Factor AP-2,
pubmed-meshheading:10405322-Transcription Factors,
pubmed-meshheading:10405322-Transcriptional Activation,
pubmed-meshheading:10405322-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Identification of the region of mi transcription factor which is responsible for the synergy with PEBP2/CBF.
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pubmed:affiliation |
Department of Pathology, Osaka University Medical School, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan. morii@patho.med.osaka-u.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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