Source:http://linkedlifedata.com/resource/pubmed/id/10404400
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
1999-9-15
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| pubmed:abstractText |
Antibodies to certain self-antigens are detected in normal individuals as well as in patients with autoimmune diseases. Natural autoantibodies found in normal human sera are thought to act as an immune regulator, a functional controller of specific proteins, or the first-line defense against pathogenic microorganisms. In the course of screening human pancreatic islet cDNA library with human sera, we found that autoantibodies to galectin-9 and its unique isoform are present in normal healthy individuals. Galectin-9 antibody was detected in all 44 human sera tested by the immunoprecipitation assays, suggesting a widespread presence of galectin-9 autoantibodies in humans. The reactivity of the sera to galectin-9 was not inhibited by lactose or endoglycosidase treatment, indicating that the reactivity was not due to a nonspecific lectin-carbohydrate interaction. We also demonstrated by RT-PCR that galectin-9 and its isoform are expressed in a variety of human tissues such as pancreatic islets, liver, lung, and tonsils as well as HeLa and Jurkat cell lines. Thus, although it has been reported previously that human galectin-9 is expressed mainly in immune cells and tissues, the current work suggests that the expression of galectin-9 and its isoform is not tissue-restricted and natural autoantibodies against them are present in normal human sera. The significance of these autoantibodies needs to be studied further.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Galectins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Isotypes,
http://linkedlifedata.com/resource/pubmed/chemical/LGALS9 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0271-9142
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
19
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
158-65
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10404400-Adult,
pubmed-meshheading:10404400-Amino Acid Sequence,
pubmed-meshheading:10404400-Autoantibodies,
pubmed-meshheading:10404400-DNA, Complementary,
pubmed-meshheading:10404400-Diabetes Mellitus, Type 1,
pubmed-meshheading:10404400-Female,
pubmed-meshheading:10404400-Galectins,
pubmed-meshheading:10404400-Gene Library,
pubmed-meshheading:10404400-Humans,
pubmed-meshheading:10404400-Immunoglobulin Isotypes,
pubmed-meshheading:10404400-Islets of Langerhans,
pubmed-meshheading:10404400-Lectins,
pubmed-meshheading:10404400-Male,
pubmed-meshheading:10404400-Middle Aged,
pubmed-meshheading:10404400-Molecular Sequence Data,
pubmed-meshheading:10404400-Precipitin Tests,
pubmed-meshheading:10404400-Protein Biosynthesis,
pubmed-meshheading:10404400-Protein Isoforms,
pubmed-meshheading:10404400-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10404400-Sequence Analysis, DNA
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| pubmed:year |
1999
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| pubmed:articleTitle |
Natural autoantibody to galectin-9 in normal human sera.
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| pubmed:affiliation |
Clinical Research Center, Samsung Biomedical Research Institute, Seoul, Korea.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|