|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1999-8-31
|
|
pubmed:abstractText |
The ends of chromosomes (telomeres) are important to maintain chromosome stability, and the loss of telomere repeat sequences has been implicated in cellular senescence and genomic instability of cancer cells. The traditional method for measuring the length of telomeres (Southern analysis) requires a large number of cells (>10(5)) and does not provide information on the telomere length of individual chromosomes. Here, we describe a digital image microscopy system for measurements of the fluorescence intensity derived from telomere repeat sequences in metaphase cells following quantitative fluorescence in situ hybridization (Q-FISH).
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0196-4763
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 1999 Wiley-Liss, Inc.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
36
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
267-78
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:10404142-Algorithms,
pubmed-meshheading:10404142-Calibration,
pubmed-meshheading:10404142-Carbocyanines,
pubmed-meshheading:10404142-DNA,
pubmed-meshheading:10404142-Humans,
pubmed-meshheading:10404142-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10404142-Indoles,
pubmed-meshheading:10404142-Karyotyping,
pubmed-meshheading:10404142-Lymphocytes,
pubmed-meshheading:10404142-Male,
pubmed-meshheading:10404142-Metaphase,
pubmed-meshheading:10404142-Microscopy, Fluorescence,
pubmed-meshheading:10404142-Particle Size,
pubmed-meshheading:10404142-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:10404142-Signal Processing, Computer-Assisted,
pubmed-meshheading:10404142-Telomere
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Telomere length measurements using digital fluorescence microscopy.
|
|
pubmed:affiliation |
Terry Fox Laboratory for Hematology/Oncology, British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
