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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-8-31
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pubmed:abstractText |
Recombinant human albumin expressed in Saccharomyces cerevisiae was compared with native human serum albumin in its physicochemical properties and in its use as a stabilizer in lyophilized preparations of thyroid-stimulating hormone (TSH), interleukin 15 (IL-15) and granulocyte colony-stimulating factor (G-CSF). Advantages of recombinant albumin include its lack of potential human contaminants and infectious agents. When used at concentrations of 0.1-0.2% (w/v), recombinant albumin was equivalent to native serum albumin in its capacity to protect immunological, biological and biochemical properties of TSH, IL-15 and G-CSF. Physicochemical characteristics of the two forms of albumin including their binding to fatty acids were also similar. The recombinant form of albumin used in this study should be considered as a suitable stabilizer in the preparation of lyophilized products and reference reagents.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1045-1056
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pubmed:author |
pubmed-author:AthaydeM LML,
pubmed-author:BolgianoBB,
pubmed-author:CorranP HPH,
pubmed-author:CraneD TDT,
pubmed-author:GeeCC,
pubmed-author:LemercinierXX,
pubmed-author:Mire-SluisAA,
pubmed-author:RaffertyBB,
pubmed-author:SutcliffeNN,
pubmed-author:TarelliEE,
pubmed-author:TivnannH AHA
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-46
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:10403037-Endotoxins,
pubmed-meshheading:10403037-Fatty Acids,
pubmed-meshheading:10403037-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:10403037-Humans,
pubmed-meshheading:10403037-Indicators and Reagents,
pubmed-meshheading:10403037-Interleukin-15,
pubmed-meshheading:10403037-Recombinant Fusion Proteins,
pubmed-meshheading:10403037-Reference Standards,
pubmed-meshheading:10403037-Serum Albumin,
pubmed-meshheading:10403037-Temperature,
pubmed-meshheading:10403037-Thyrotropin
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pubmed:year |
1998
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pubmed:articleTitle |
Recombinant human albumin as a stabilizer for biological materials and for the preparation of international reference reagents.
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pubmed:affiliation |
Division of Chemistry, National Institute for Biological Standards and Control, Potters Bar, Herts, U.K.
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pubmed:publicationType |
Journal Article
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