Linked Life Data

10398845

Source:http://linkedlifedata.com/resource/pubmed/id/10398845

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1999-9-10
pubmed:abstractText
The chromatin protein NonA from Drosophila, present in many puffs on polytene chromosomes, belongs to the growing class of RRM proteins. Exchange of amino acids within the RNP1 and RNP2 consensus sequences, known from other RRM proteins to be essential for RNA binding, has been shown drastically to reduce NonA function in vivo. Here we compare NonA binding to RNA from the Sgs-4 gene, an in situ target for NonA, with binding to Sgs-3 RNA, which is not a target of NonA. Using an immunoprecipitation assay in vitro we show that NonA binds to single-stranded (ss)DNA and RNA with moderate affinity (KD=8x10(-8) M). However, we did not observe sequence-specific binding to the Sgs-4 transcript nor to Sgs-4 DNA containing upstream regulatory sequences. Point mutations within the RNP1 and RNP2 consensus sequences that interfere with NonA function in vivo do not significantly change chromosomal binding nor the general affinity for RNA. The expression of Sgs-4 RNA relative to the expression of Sgs-3 RNA remains the same in the presence or absence of NonA protein. com/link/service/journals/00412/bibs/108n3p162.html
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0009-5915
pubmed:author
pubmed:issnType
Print
pubmed:volume
108
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
162-72
pubmed:dateRevised
2008-10-16
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
The puff-specific RRM protein NonA is a single-stranded nucleic acid binding protein.
pubmed:affiliation
Institut für Biologie, Abt. Zytogenetik, Humboldt Universität zu Berlin, Chausseestrasse 117, D-10115 Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't