Linked Life Data

10397910

Source:http://linkedlifedata.com/resource/pubmed/id/10397910

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-10-2
pubmed:abstractText
Nanoparticles of poly(lactic acid) (PLA) and of blends of PLA and monomethoxypoly(ethylene oxyde) (MPEO-PLA) were prepared by the double emulsion method. Sucrose was added to overcome nanoparticle aggregation with freeze-drying. Whereas PLA nanoparticles rapidly are phagocytosed by the mononuclear phagocyte system cells, the uptake of MPEO-PLA nanoparticles is delayed. Opsonization is one of the steps of phagocytosis, and serum complement is a major component of the opsonin system. The in vitro complement consumption of the prepared nanoparticles was evaluated as a function of time and of their surface area. To avoid the aggregation of MPEO-PLA nanoparticles due to MPEO crystallization, sucrose was necessary, and its concentration was dependent on MPEO proportion in the nanoparticle suspension. As expected, the complement consumption for PLA nanoparticles is faster and more important than for PLA/MPEO-PLA blends. The complement consumption decreases with the increase in MPEO surface density. Complement is less consumed with the lower surface density provided by a higher molecular weight than by the higher surface density provided by a lower molecular weight MPEO. The complement consumption seems to be dependent on the number of nanoparticles in contact with human serum. Finally, the model of steric repulsion of MPEO towards proteins and the importance of the surface density of MPEO were emphasized.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9304
pubmed:author
pubmed:copyrightInfo
Copyright 1999 John Wiley & Sons, Inc.
pubmed:issnType
Print
pubmed:volume
44
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-15
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
MPEO-PLA nanoparticles: effect of MPEO content on some of their surface properties.
pubmed:affiliation
Laboratoire d'Hématologie-Physiologie, Faculté de Pharmacie, 5, rue Albert Lebrun, 54001 Nancy Cedex, France.
pubmed:publicationType
Journal Article