Source:http://linkedlifedata.com/resource/pubmed/id/10397707
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1999-8-5
|
| pubmed:abstractText |
The malignant Reed-Sternberg cell of Hodgkin's disease, first described a century ago, has resisted in-depth analysis due to its extreme rarity in lymphomatous tissue. To directly study its genome-wide gene expression, approximately 11,000,000 bases (27,518 cDNA sequences) of expressed gene sequence was determined from living single Reed-Sternberg cells, Hodgkin's tissue, and cell lines. This approach increased the number of genes known to be expressed in Hodgkin's disease by 20-fold to 2,666 named genes. The data here indicate that Reed-Sternberg cells from both nodular sclerosing and lymphocyte predominant Hodgkin's disease were derived from an unusual B-cell lineage based on a comparison of their gene expression to approximately 40,000,000 bases (10(5) sequences) of expressed gene sequence from germinal center B cells (GCB) and dendritic cells. The data set of expressed genes, reported here and on the World Wide Web, forms a basis to understand the genes responsible for Hodgkin's disease and develop novel diagnostic markers and therapies. This study of the rare Reed-Sternberg cell, concealed in its heterogenous cellular context, also provides a formidable test case to advance the limit of analysis of differential gene expression to the single disease cell.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
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| pubmed:volume |
94
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
411-6
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10397707-B-Lymphocytes,
pubmed-meshheading:10397707-Cell Differentiation,
pubmed-meshheading:10397707-Cell Lineage,
pubmed-meshheading:10397707-DNA, Complementary,
pubmed-meshheading:10397707-Dendritic Cells,
pubmed-meshheading:10397707-Expressed Sequence Tags,
pubmed-meshheading:10397707-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:10397707-Gene Library,
pubmed-meshheading:10397707-Germinal Center,
pubmed-meshheading:10397707-Hodgkin Disease,
pubmed-meshheading:10397707-Humans,
pubmed-meshheading:10397707-Neoplasm Proteins,
pubmed-meshheading:10397707-RNA, Messenger,
pubmed-meshheading:10397707-RNA, Neoplasm,
pubmed-meshheading:10397707-Reed-Sternberg Cells,
pubmed-meshheading:10397707-Sequence Homology, Nucleic Acid,
pubmed-meshheading:10397707-Tumor Cells, Cultured
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Reed-Sternberg cell genome expression supports a B-cell lineage.
|
| pubmed:affiliation |
Georgetown University Medical Center, Washington, DC, USA. cossmanj@gunet.georgetown.edu
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|