Linked Life Data

10393431

Source:http://linkedlifedata.com/resource/pubmed/id/10393431

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3-4
pubmed:dateCreated
1999-7-22
pubmed:databankReference
pubmed:abstractText
Many eukaryotic cell surface proteins are anchored to the membrane with glycosylphosphatidylinositol (GPI) that is covalently linked to the carboxyl-terminus. A Saccharomyces cerevisiae gaa1 mutant is defective in posttranslational attachment of GPI to proteins. A recent report demonstrated that the GPAA1 gene encodes a component of a transamidase that mediates GPI-anchor attachment. Here, we report structures and chromosome loci of human and mouse GPAA1 genes. Both genes consist of twelve exons that span about 4 kb. Human and mouse GPAA1s are located at 8q24.3 and 15E, respectively. There is a human pseudo GPAA1 gene (GPAA1P1) that is located at 2q12-->q14. Introns 8 of human and mouse GPAA1s were minor class introns bearing AT at the 5' splice sites and AC and AT at the 3' splice sites, respectively. The 3' splice sites of corresponding introns of African green monkey, Chinese hamster, dog and rat were AC, AT, AT and AA, respectively. The mouse GPAA1 gene (Gpaa1) bearing AG at the 3' splice site prepared by site-directed mutagenesis was functional, indicating that any nucleotide is allowed at the 3' end of a minor class intron.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0301-0171
pubmed:author
pubmed:issnType
Print
pubmed:volume
84
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
199-205
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10393431-Animals, pubmed-meshheading:10393431-Antigens, Thy-1, pubmed-meshheading:10393431-Base Sequence, pubmed-meshheading:10393431-Cell Line, pubmed-meshheading:10393431-Chromosomes, pubmed-meshheading:10393431-Chromosomes, Human, Pair 2, pubmed-meshheading:10393431-Chromosomes, Human, Pair 8, pubmed-meshheading:10393431-Cloning, Molecular, pubmed-meshheading:10393431-Exons, pubmed-meshheading:10393431-Genome, pubmed-meshheading:10393431-Humans, pubmed-meshheading:10393431-Introns, pubmed-meshheading:10393431-Membrane Glycoproteins, pubmed-meshheading:10393431-Mice, pubmed-meshheading:10393431-Molecular Sequence Data, pubmed-meshheading:10393431-Physical Chromosome Mapping, pubmed-meshheading:10393431-Proteins, pubmed-meshheading:10393431-Pseudogenes, pubmed-meshheading:10393431-RNA, Messenger, pubmed-meshheading:10393431-RNA Splicing, pubmed-meshheading:10393431-Saccharomyces cerevisiae Proteins, pubmed-meshheading:10393431-Transfection
pubmed:year
1999
pubmed:articleTitle
Human and mouse GPAA1 (Glycosylphosphatidylinositol anchor attachment 1) genes: genomic structures, chromosome loci and the presence of a minor class intron.
pubmed:affiliation
Department of Immunoregulation, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't