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rdf:type |
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lifeskim:mentions |
umls-concept:C0003250,
umls-concept:C0006141,
umls-concept:C0017262,
umls-concept:C0078671,
umls-concept:C0185117,
umls-concept:C0205314,
umls-concept:C0205369,
umls-concept:C0597298,
umls-concept:C0679622,
umls-concept:C1140680,
umls-concept:C1417490,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
1999-7-19
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pubmed:abstractText |
The products of the MUC1 gene are known to be highly expressed in human breast cancer cells. The best characterized MUC1 protein is a polymorphic, type 1 transmembrane molecule containing a large extracellular domain composed primarily of a variable number of 20 amino acid tandem repeats. We have recently identified a novel protein product of the MUC1 gene, the MUC1/Y protein, that is also a transmembrane protein but is devoid of the tandem repeat array and its immediate flanking sequences. To analyze its expression in tumor cells we generated monoclonal antibodies directed against the MUC1/Y extracellular domain (anti-MUC1/Yex MAbs). Epitope mapping identified the MAb, 6E6, which recognized the MUC1/Y isoform with exquisite specificity- the repeat-array-containing MUC1 isoform could not compete out this immunoreactivity. A 30mer peptide which is unique for MUC1/Y and corresponds to the "join" region generated by the MUC1/Y specific splice, abrogated all 6E6 MAb immunoreactivity towards MUC1/Y. Immunoprecipitation of the MUC1/Y protein with 6E6 MAbs revealed that, in contrast with the proteolytic cleavage of the tandem-repeat-array-containing MUC1 isoform, MUC1/Y is not cleaved. Flow cytometry analyses using the 6E6 MAbs demonstrated that the MUC1/Y isoform is expressed on the cell surface of both MCF-7 breast cancer cells and malignant epithelial cells present in effusions obtained from breast and ovarian cancer patients. Our results unequivocally establish that the MUC1/Y protein is expressed on the surface of breast cancer cells and cells of other epithelial malignancies. The anti-MUC1/Y MAbs described here can target MUC1/Y expressing tumor cells in vivo and are likely to be important reagents both for epithelial tumor diagnosis and immunotherapy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Mucin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0020-7136
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pubmed:author |
pubmed-author:AderetYY,
pubmed-author:BaruchAA,
pubmed-author:GreensteinSS,
pubmed-author:HarnessEE,
pubmed-author:HartmanMM,
pubmed-author:KeydarII,
pubmed-author:RoyDD,
pubmed-author:Sagi-AssifOO,
pubmed-author:SmorodinskyN INI,
pubmed-author:StadlerYY,
pubmed-author:WeissMM,
pubmed-author:WreschnerD HDH,
pubmed-author:YaakubovitsMM,
pubmed-author:YoeliMM
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
256-67
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10389761-3T3 Cells,
pubmed-meshheading:10389761-Animals,
pubmed-meshheading:10389761-Antibodies, Monoclonal,
pubmed-meshheading:10389761-Ascites,
pubmed-meshheading:10389761-Breast Neoplasms,
pubmed-meshheading:10389761-DNA, Complementary,
pubmed-meshheading:10389761-Epithelial Cells,
pubmed-meshheading:10389761-Epitopes,
pubmed-meshheading:10389761-Female,
pubmed-meshheading:10389761-Flow Cytometry,
pubmed-meshheading:10389761-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:10389761-Humans,
pubmed-meshheading:10389761-Mammary Neoplasms, Experimental,
pubmed-meshheading:10389761-Mice,
pubmed-meshheading:10389761-Mice, Inbred BALB C,
pubmed-meshheading:10389761-Mucin-1,
pubmed-meshheading:10389761-Neoplasm Proteins,
pubmed-meshheading:10389761-Ovarian Neoplasms,
pubmed-meshheading:10389761-Pleural Effusion, Malignant,
pubmed-meshheading:10389761-Protein Isoforms,
pubmed-meshheading:10389761-Protein Structure, Secondary,
pubmed-meshheading:10389761-RNA, Messenger,
pubmed-meshheading:10389761-RNA, Neoplasm,
pubmed-meshheading:10389761-RNA Splicing,
pubmed-meshheading:10389761-Recombinant Fusion Proteins,
pubmed-meshheading:10389761-Transfection,
pubmed-meshheading:10389761-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
MUC1 isoform specific monoclonal antibody 6E6/2 detects preferential expression of the novel MUC1/Y protein in breast and ovarian cancer.
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pubmed:affiliation |
Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, Israel.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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