Linked Life Data

10381543

Source:http://linkedlifedata.com/resource/pubmed/id/10381543

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-9-21
pubmed:abstractText
The regulation of mu-opioid receptor gene expression was investigated using several molecular techniques. Genomic clones containing portions of the human mu-opioid receptor gene were sequenced. 5'-RACE analysis of human brain cDNA confirmed the presence of mRNAs up to -313 from the start codon. As was found for the mouse and rat genes, transcription apparently initiates in the absence of a discernable TATA box. To characterize promoter function, portions of the 5'-flanking region were linked to a reporter gene in transient transfection experiments. Two approximately 50 bp adjacent segments had potent, orientation specific promoter activity. More down-stream segments also had promoter activity. None of the 5'-flanking region constructs showed tissue specificity. The potential role of DNA methylation in preventing ectopic expression was investigated by surveying the methylation state of a CpG rich region straddling the start codon. A neural derived cell line (SH-SY5Y) that expresses the mu-opioid receptor lacked virtually any CpG methylation. In contrast, two neural derived cell lines that do not express the mu-opioid receptor were nearly totally methylated while non-neural cell lines had intermediate levels of CpG methylation. Additional transient transfection experiments revealed that CpG methylation of the 5'-flanking region suppressed reporter gene expression. These results indicate that CpG methylation plays an important role in regulating mu-opioid receptor expression in neural cells; however, no association was found with regulation of expression in non-neural cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0169-328X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Published By Elsevier Science B.V.
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
54-65
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10381543-Animals, pubmed-meshheading:10381543-Carcinoma, Squamous Cell, pubmed-meshheading:10381543-CpG Islands, pubmed-meshheading:10381543-DNA, Complementary, pubmed-meshheading:10381543-DNA Methylation, pubmed-meshheading:10381543-Gene Expression Regulation, pubmed-meshheading:10381543-Genes, Reporter, pubmed-meshheading:10381543-Humans, pubmed-meshheading:10381543-Kidney, pubmed-meshheading:10381543-L Cells (Cell Line), pubmed-meshheading:10381543-Luciferases, pubmed-meshheading:10381543-Mice, pubmed-meshheading:10381543-Nerve Tissue Proteins, pubmed-meshheading:10381543-Neuroblastoma, pubmed-meshheading:10381543-Organ Specificity, pubmed-meshheading:10381543-Polymerase Chain Reaction, pubmed-meshheading:10381543-Promoter Regions, Genetic, pubmed-meshheading:10381543-Rats, pubmed-meshheading:10381543-Receptors, Opioid, mu, pubmed-meshheading:10381543-Recombinant Fusion Proteins, pubmed-meshheading:10381543-Transcription, Genetic, pubmed-meshheading:10381543-Transfection, pubmed-meshheading:10381543-Tumor Cells, Cultured
pubmed:year
1999
pubmed:articleTitle
Localization of promoter elements in the human mu-opioid receptor gene and regulation by DNA methylation.
pubmed:affiliation
Department of Psychiatry, New York University Medical Center, Millhauser Labs HN605, New York, NY 10016, USA. andrim01@mcrcr.med.nyu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.