| pubmed-article:10376974 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C1519025 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C0007600 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C0021084 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C1883036 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C1414262 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C2348358 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C1707391 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C1708111 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C1720127 | lld:lifeskim |
| pubmed-article:10376974 | lifeskim:mentions | umls-concept:C0072544 | lld:lifeskim |
| pubmed-article:10376974 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:10376974 | pubmed:dateCreated | 1999-6-30 | lld:pubmed |
| pubmed-article:10376974 | pubmed:abstractText | The human CD30 receptor is highly overexpressed on the surface of Hodgkin Reed-Sternberg cells and has been shown to be an excellent target for selective immunotherapy using monoclonal antibody-based agents such as immunotoxins. To construct a new recombinant immunotoxin for possible clinical use in patients with Hodgkin's lymphoma, we have chosen the murine anti-CD30 hybridoma Ki-4 to generate a high-affinity Ki-4 single-chain variable fragment (scFv). Hybridoma V-genes were polymerase chain reaction-amplified, assembled, cloned and expressed as a mini-library for display on filamentous phage. Functional Ki-4 scFv were obtained by selection of binding phage on the Hodgkin lymphoma-derived, CD30-expressing cell line L540Cy. The selected recombinant Ki-4 scFv was shown to specifically bind to an overlapping epitope on the CD30 antigen with binding kinetics similar to those of the original antibody. The Ki-4 scFv was subsequently fused to a deletion mutant of Pseudomonas exotoxin A (ETA'). The resulting immunotoxin Ki-4(scFv)-ETA' specifically binds to CD30+ L540Cy cells and inhibits the protein synthesis by 50% at a concentration (IC50) of 43 pM. This recombinant immunotoxin is a promising candidate for further clinical evaluation in patients with Hodgkin's lymphoma or other CD30+ malignancies. | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10376974 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10376974 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10376974 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10376974 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:10376974 | pubmed:issn | 0007-0920 | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:DiehlVV | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:BartaTT | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:LemkeHH | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:MatthesHH | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:HansenHH | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:EngertAA | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:HoogenboomH... | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:ArendsJ WJW | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:KlimkeEE | lld:pubmed |
| pubmed-article:10376974 | pubmed:author | pubmed-author:RooversR CRC | lld:pubmed |
| pubmed-article:10376974 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10376974 | pubmed:volume | 80 | lld:pubmed |
| pubmed-article:10376974 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10376974 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10376974 | pubmed:pagination | 1214-22 | lld:pubmed |
| pubmed-article:10376974 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:meshHeading | pubmed-meshheading:10376974... | lld:pubmed |
| pubmed-article:10376974 | pubmed:year | 1999 | lld:pubmed |
| pubmed-article:10376974 | pubmed:articleTitle | An anti-CD30 single-chain Fv selected by phage display and fused to Pseudomonas exotoxin A (Ki-4(scFv)-ETA') is a potent immunotoxin against a Hodgkin-derived cell line. | lld:pubmed |
| pubmed-article:10376974 | pubmed:affiliation | Department of Internal Medicine I, University Hospital Cologne, Germany. | lld:pubmed |
| pubmed-article:10376974 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10376974 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376974 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376974 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376974 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376974 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376974 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376974 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10376974 | lld:pubmed |
