|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
14
|
|
pubmed:dateCreated |
1999-8-19
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
XTcf-3 is an HMG box transcription factor that mediates Xenopus dorsal-ventral axis formation. As a Wnt pathway effector, XTcf-3 interacts with beta-catenin and activates the expression of the dorsal organizing gene siamois, while in the absence of beta-catenin, XTcf-3 functions as a transcriptional repressor. We show that XTcf-3 contains amino- and carboxy-terminal repressor domains and have identified a Xenopus member of the C-terminal Binding Protein family of transcriptional co-repressors (XCtBP) as the C-terminal co-repressor. We show that two XCtBP binding sites near the XTcf-3 carboxy-terminus are required for the interaction of XTcf-3 and XCtBP and for the transcriptional repression mediated by the XTcf-3 carboxy-terminal domain. By fusing the GAL4 activation domain to XCtBP we have generated an antimorphic protein, XCtBP/G4A, that activates siamois transcription through an interaction with endogenous XTcf-3. Ectopic expression of XCtBP/G4A demonstrates that XCtBP functions in the regulation of head and notochord development. Our data support a role for XCtBP as a co-repressor throughout Xenopus development and indicate that XCtBP/G4A will be a useful tool in determining how XCtBP functions in various developmental processes.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HMGB Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/High Mobility Group Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphoid Enhancer-Binding Factor 1,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/TCF Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tcf3 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Thyroid Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor 3,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor 7-Like 1...,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/XLef-1 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/siamois protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/thyroid hormone-binding proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0950-1991
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
126
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
3159-70
|
|
pubmed:dateRevised |
2010-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10375506-Amino Acid Sequence,
pubmed-meshheading:10375506-Animals,
pubmed-meshheading:10375506-Binding Sites,
pubmed-meshheading:10375506-Carrier Proteins,
pubmed-meshheading:10375506-Conserved Sequence,
pubmed-meshheading:10375506-Embryo, Nonmammalian,
pubmed-meshheading:10375506-Gene Expression Regulation, Developmental,
pubmed-meshheading:10375506-HMGB Proteins,
pubmed-meshheading:10375506-High Mobility Group Proteins,
pubmed-meshheading:10375506-Homeodomain Proteins,
pubmed-meshheading:10375506-Hybrid Cells,
pubmed-meshheading:10375506-Lymphoid Enhancer-Binding Factor 1,
pubmed-meshheading:10375506-Membrane Proteins,
pubmed-meshheading:10375506-Molecular Sequence Data,
pubmed-meshheading:10375506-Recombinant Proteins,
pubmed-meshheading:10375506-Repressor Proteins,
pubmed-meshheading:10375506-Sequence Homology, Amino Acid,
pubmed-meshheading:10375506-TCF Transcription Factors,
pubmed-meshheading:10375506-Thyroid Hormones,
pubmed-meshheading:10375506-Transcription, Genetic,
pubmed-meshheading:10375506-Transcription Factor 3,
pubmed-meshheading:10375506-Transcription Factor 7-Like 1 Protein,
pubmed-meshheading:10375506-Transcription Factors,
pubmed-meshheading:10375506-Xenopus,
pubmed-meshheading:10375506-Xenopus Proteins,
pubmed-meshheading:10375506-Yeasts
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
XCtBP is a XTcf-3 co-repressor with roles throughout Xenopus development.
|
|
pubmed:affiliation |
Department of Biochemistry, Howard Hughes Medical Institute, Center for Developmental Biology, University of Washington School of Medicine, Seattle, Washington 98195-7350, USA. kimelman@u.washington.edu.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
