Linked Life Data

10373466

Source:http://linkedlifedata.com/resource/pubmed/id/10373466

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
26
pubmed:dateCreated
1999-7-15
pubmed:databankReference
pubmed:abstractText
We determined the complete nucleotide sequences of the alpha1 subunit gene (OlGCS-alpha1) and the beta1 subunit gene (OlGCS-beta1) of medaka fish soluble guanylyl cyclase. In the genome, OlGCS-alpha1 and OlGCS-beta1 are organized in tandem. The two genes are only 986 base pairs apart and span approximately 34 kilobase pairs in the order of OlGCS-alpha1 and OlGCS-beta1. The nucleotide sequence of a large part of the 5'-upstream region of OlGCS-alpha1 is complimentarily conserved in that of OlGCS-beta1. To analyze the promoter activity of each gene, a fusion gene construct in which the 5'-upstream region was fused with the green fluorescent protein gene was injected into medaka fish 2-cell embryos. When the fusion gene containing the OlGCS-alpha1 upstream region was injected, green fluorescent protein fluorescence was detected in the embryonic brain. The 5'-upstream region of OlGCS-beta1 alone was insufficient for the reporter gene expression in the embryos. When the OlGCS-alpha1 upstream region was located upstream of the OlGCS-beta1-green fluorescence protein fusion gene, the reporter gene was expressed in the brain and trunk region of the embryos. These results suggest that the 5'-upstream region of OlGCS-alpha1 can affect the expression of OlGCS-beta1. It is therefore possible that the expression of OlGCS-alpha1 and OlGCS-beta1 is coordinated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
18567-73
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Tandem organization of medaka fish soluble guanylyl cyclase alpha1 and beta1 subunit genes. Implications for coordinated transcription of two subunit genes.
pubmed:affiliation
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't