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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
1999-7-23
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pubmed:abstractText |
The rhesus rhadinovirus strain 17577 (RRV strain 17577) genome is essentially colinear with human herpesvirus 8 (HHV8)/Kaposi's sarcoma-associated herpesvirus (KSHV) and encodes several analogous open reading frames (ORFs), including the homologue of cellular interleukin-6 (IL-6). To determine if the RRV IL-6-like ORF (RvIL-6) is biologically functional, it was expressed either transiently in COS-1 cells or purified from bacteria as a glutathione S-transferase (GST)-RvIL-6 fusion and analyzed by IL-6 bioassays. Utilizing the IL-6-dependent B9 cell line, we found that both forms of RvIL-6 supported cell proliferation in a dose-dependent manner. Moreover, antibodies specific to the IL-6 receptor (IL-6R) or the gp130 subunit were capable of blocking the stimulatory effects of RvIL-6. Reciprocal titrations of GST-RvIL-6 against human recombinant IL-6 produced a more-than-additive stimulatory effect, suggesting that RvIL-6 does not inhibit but may instead potentiate normal cellular IL-6 signaling to B cells. These results demonstrate that RRV encodes an accessory protein with IL-6-like activity.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-10074154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-1315587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-2161559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-2788034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-3500054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7559730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7561102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7609088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7632932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7700310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7700311,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7714331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7773741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-7891487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-8083235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-8276883,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-8877726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-8939871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9055855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9118960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9144766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9235971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9365244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9371642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9490667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10364379-9842891
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
73
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6177-81
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10364379-Amino Acid Sequence,
pubmed-meshheading:10364379-Animals,
pubmed-meshheading:10364379-Antigens, CD,
pubmed-meshheading:10364379-Cell Line,
pubmed-meshheading:10364379-Cytokine Receptor gp130,
pubmed-meshheading:10364379-Herpesvirus 8, Human,
pubmed-meshheading:10364379-Humans,
pubmed-meshheading:10364379-Interleukin-6,
pubmed-meshheading:10364379-Macaca mulatta,
pubmed-meshheading:10364379-Membrane Glycoproteins,
pubmed-meshheading:10364379-Molecular Sequence Data,
pubmed-meshheading:10364379-Receptors, Interleukin-6,
pubmed-meshheading:10364379-Recombination, Genetic,
pubmed-meshheading:10364379-Rhadinovirus,
pubmed-meshheading:10364379-Sarcoma, Kaposi,
pubmed-meshheading:10364379-Viral Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
A rhesus macaque rhadinovirus related to Kaposi's sarcoma-associated herpesvirus/human herpesvirus 8 encodes a functional homologue of interleukin-6.
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pubmed:affiliation |
Division of Pathobiology and Immunology, Oregon Regional Primate Research Center, Beaverton, Oregon 97006, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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