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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1999-6-24
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pubmed:abstractText |
Peptides derived from the melanoma-associated MART-1/Melan-A antigen are currently implemented in immunotherapy for inducing or augmenting T-cell responses directed against peptides expressed by autologous tumor cells in HLA-A2+ patients with melanoma. Here, we describe the specificity of the T-cell clone SK29-FFM1.1, which secretes GM-CSF in response to a panel of synthetic MART-1/Melan-A-derived peptides, including the naturally presented ILTVILGVL(32-40), but exhibits cytotoxicity and IFN-gamma secretion exclusively to the MART-1/Melan-A derived peptide AAGIGILTV(27-35). In addition, cytotoxic T-lymphocyte (CTL) clone SK29-FFM1.1 recognizes 3 different naturally processed and presented peptides on HLA-A2+ MART-1/Melan-A+ melanoma cells, as defined by cytotoxicity and IFN-gamma and GM-CSF secretion. Processing and presentation of MART-1/Melan-A peptides appears to be different in cells of non-melanocytic origin, as shown by the characterization of naturally presented peptides displayed by HLA-A2+ colorectal cancer cells transduced with a MART-1/Melan-A gene-containing retrovirus. Our data suggest that multiple epitopes, including ILTVILGVL and different isoforms of AAGIGILTV derived from MART-1/Melan-A may be naturally presented by melanoma cells to the immune system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-A2 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0020-7136
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
81
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
979-84
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:10362148-Amino Acid Sequence,
pubmed-meshheading:10362148-Antigens, Neoplasm,
pubmed-meshheading:10362148-Clone Cells,
pubmed-meshheading:10362148-Cytotoxicity, Immunologic,
pubmed-meshheading:10362148-HLA-A2 Antigen,
pubmed-meshheading:10362148-Humans,
pubmed-meshheading:10362148-Interferon-gamma,
pubmed-meshheading:10362148-MART-1 Antigen,
pubmed-meshheading:10362148-Melanoma,
pubmed-meshheading:10362148-Neoplasm Proteins,
pubmed-meshheading:10362148-Protein Isoforms,
pubmed-meshheading:10362148-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:10362148-Recombinant Proteins,
pubmed-meshheading:10362148-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10362148-T-Lymphocytes,
pubmed-meshheading:10362148-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:10362148-Transfection,
pubmed-meshheading:10362148-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Cytotoxic T lymphocytes define multiple peptide isoforms derived from the melanoma-associated antigen MART-1/Melan-A.
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pubmed:affiliation |
Medizinische Klinik II, Hämatologie-Onkologie, Krankenhaus Nordwest, Frankfurt, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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