Linked Life Data

10357842

Source:http://linkedlifedata.com/resource/pubmed/id/10357842

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1999-7-15
pubmed:abstractText
Oxidation of lipoproteins is thought to be an early event in atherogenesis. To evaluate whether aortic lipoprotein lipid (per)oxidation contributes to atherosclerosis, we investigated the time-dependent changes to lipids and antioxidants in plasma and aortas of apolipoprotein E gene knockout (apoE-/-) mice receiving a high fat diet, and compared these changes with lesion development. Circulating buoyant lipoproteins and associated cholesterol (C), cholesteryl esters (CE), and alpha-tocopherol (alpha-TOH) increased within 1 month then remained largely constant up to 6 months. Coenzyme Q (CoQ) remained unchanged for the first 3 months and increased marginally after 6 months. With increasing duration of the diet, plasma lipids showed an increased propensity to undergo peroxyl radical-induced (per)oxidation. Absolute concentrations of aortic C, hydroperoxides and hydroxides of CE (CE-O(O)H) and alpha-TOH increased gradually while aortic CE increased more markedly with changes to cholesteryl linoleate being most pronounced. Aortic CoQ remained largely unchanged. Overall, the extent of aortic CE (per)oxidation remained low (</=1%) and the ratio of incremental changes of alpha-TOH to oxidizable lipid remained unchanged. Aortic biochemistry paralleled lesion formation, particularly that in the descending thoracic aorta.Together, our results show that progressing atherosclerosis in apoE-/- mice is associated with increased aortic lipid (per)oxidation as assessed by the concentrations of CE-O(O)H, measured directly by HPLC. This supports the oxidation theory. Measurement of aortic CE-O(O)H may be useful for mechanistic studies studying the relationship between inhibition of in vivo lipid (per)oxidation and atherosclerosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-2275
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1104-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10357842-Animals, pubmed-meshheading:10357842-Antioxidants, pubmed-meshheading:10357842-Aorta, pubmed-meshheading:10357842-Apolipoproteins E, pubmed-meshheading:10357842-Arteriosclerosis, pubmed-meshheading:10357842-Cholesterol Esters, pubmed-meshheading:10357842-Chromatography, High Pressure Liquid, pubmed-meshheading:10357842-Dietary Fats, pubmed-meshheading:10357842-Kinetics, pubmed-meshheading:10357842-Lipid Metabolism, pubmed-meshheading:10357842-Lipid Peroxidation, pubmed-meshheading:10357842-Lipids, pubmed-meshheading:10357842-Lipoproteins, pubmed-meshheading:10357842-Male, pubmed-meshheading:10357842-Mice, pubmed-meshheading:10357842-Mice, Inbred C57BL, pubmed-meshheading:10357842-Mice, Knockout, pubmed-meshheading:10357842-Peroxides, pubmed-meshheading:10357842-Ubiquinone, pubmed-meshheading:10357842-Vitamin E
pubmed:year
1999
pubmed:articleTitle
Time-dependent changes to lipids and antioxidants in plasma and aortas of apolipoprotein E knockout mice.
pubmed:affiliation
Biochemistry Group, Heart Research Institute, 145 Missenden Road, Camperdown, 2050, Sydney, NSW, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't