|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
1999-7-27
|
|
pubmed:abstractText |
A Cycling Probe Technology (CPT) assay was developed for the detection of the mecA gene from methicillin resistant staphylococcal cultures. The assay is based on a colorimetric enzyme-immuno-assay (EIA) and uses a mecA probe (DNA-RNA-DNA) labeled with fluorescein at the 5'-terminus and biotin at the 3'-terminus. The reaction occurs at a constant temperature that allows the target DNA to anneal to the probe. RNase H cuts the RNA portion, allowing the cut fragments to dissociate from the target, making it available for further cycling. CPT-EIA uses streptavidin-coated microplate wells to capture uncut probe followed by detection with horseradish-peroxidase conjugated anti-fluorescein antibody. The assay was compared to PCR and shown to accurately detect the presence or absence of the mecA gene in 159 staphylococcal clinical isolates. The CPT-EIA assay takes two hours starting from cultured cells compared with the 24-48 h required for detection of methicillin resistance by conventional susceptibility tests.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
0732-8893
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
34
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
83-90
|
|
pubmed:dateRevised |
2007-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:10354856-Bacterial Proteins,
pubmed-meshheading:10354856-Carrier Proteins,
pubmed-meshheading:10354856-Colorimetry,
pubmed-meshheading:10354856-Genes, Bacterial,
pubmed-meshheading:10354856-Hexosyltransferases,
pubmed-meshheading:10354856-Humans,
pubmed-meshheading:10354856-Immunoenzyme Techniques,
pubmed-meshheading:10354856-Methicillin,
pubmed-meshheading:10354856-Methicillin Resistance,
pubmed-meshheading:10354856-Molecular Probe Techniques,
pubmed-meshheading:10354856-Muramoylpentapeptide Carboxypeptidase,
pubmed-meshheading:10354856-Penicillin-Binding Proteins,
pubmed-meshheading:10354856-Penicillins,
pubmed-meshheading:10354856-Peptidyl Transferases,
pubmed-meshheading:10354856-Polymerase Chain Reaction,
pubmed-meshheading:10354856-Ribonuclease H,
pubmed-meshheading:10354856-Staphylococcal Infections,
pubmed-meshheading:10354856-Staphylococcus
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Rapid detection of the mecA gene in methicillin resistant staphylococci using a colorimetric cycling probe technology.
|
|
pubmed:affiliation |
ID Biomedical Corporation, Burnaby, B.C., Canada.
|
|
pubmed:publicationType |
Journal Article
|
