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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1999-7-12
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pubmed:abstractText |
Because Mg2+ and Li+ ions have similar chemical properties, we have hypothesized that Li+/Mg2+ competition for Mg2+ binding sites is the molecular basis for the therapeutic action of lithium in manic-depressive illness. By fluorescence spectroscopy with furaptra-loaded cells, the free intracellular Mg2+ concentration within the intact neuroblastoma cells was found to increase from 0. 39 +/- 0.04 mM to 0.60 +/- 0.04 mM during a 40-min Li+ incubation in which the total intracellular Li+ concentration increased from 0 to 5.5 mM. Our fluorescence microscopy observations of Li+-free and Li+-loaded cells also indicate an increase in free Mg2+ concentration upon Li+ incubation. By 31P NMR, the free intracellular Mg2+ concentrations for Li+-free cells was 0.35 +/- 0. 03 mM and 0.80 +/- 0.04 mM for Li+-loaded cells (final total intracellular Li+ concentration of 16 mM). If a Li+/Mg2+ competition mechanism is present in neuroblastoma cells, an increase in the total intracellular Li+ concentration is expected to result in an increase in the free intracellular Mg2+ concentration, because Li+ displaces Mg2+ from its binding sites within the nerve cell. The fluorescence spectroscopy, fluorescence microscopy, and 31P NMR spectroscopy studies presented here have shown this to be the case.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-1402889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-1514577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-1562190,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-1880501,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-2191781,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-2342403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-2553271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-2923192,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-2924906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-3119368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-3340189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-423756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-4748425,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-5288124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-6479241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-7620702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-7700249,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-7834216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-8097884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-8135827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-8155627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-8264511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-8455027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-8789387,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-8842030,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-958476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10354421-9751202
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3495
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2934-42
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10354421-Binding, Competitive,
pubmed-meshheading:10354421-Binding Sites,
pubmed-meshheading:10354421-Biophysical Phenomena,
pubmed-meshheading:10354421-Biophysics,
pubmed-meshheading:10354421-Bipolar Disorder,
pubmed-meshheading:10354421-Calcium,
pubmed-meshheading:10354421-Fluorescent Dyes,
pubmed-meshheading:10354421-Fura-2,
pubmed-meshheading:10354421-Humans,
pubmed-meshheading:10354421-Lithium,
pubmed-meshheading:10354421-Magnesium,
pubmed-meshheading:10354421-Magnetic Resonance Spectroscopy,
pubmed-meshheading:10354421-Microscopy, Fluorescence,
pubmed-meshheading:10354421-Neuroblastoma,
pubmed-meshheading:10354421-Neurons,
pubmed-meshheading:10354421-Spectrometry, Fluorescence,
pubmed-meshheading:10354421-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Competition between Li+ and Mg2+ in neuroblastoma SH-SY5Y cells: a fluorescence and 31P NMR study.
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pubmed:affiliation |
Department of Chemistry, Loyola University, Chicago, Illinois 60626 USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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