Linked Life Data

10334864

Source:http://linkedlifedata.com/resource/pubmed/id/10334864

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-6-24
pubmed:abstractText
We previously developed a simple competitive reaction model between lipid peroxidation and protein oxidation in erythrocyte membranes that accounts for radical-induced hemolysis of human erythrocytes. In this study, we compared the rate constants calculated from the hemolysis curves of erythrocytes in the presence of radical initiators with those obtained from experiments using erythrocyte ghosts treated with radicals. 2,2'-Azobis(amidinopropane) dihydrochloride and 2,2'-azobis(2,4-dimethylvaleronitrile) were used as radical initiators. Plots of the logarithm of concentration of the radical initiator against the logarithm of the rate constant gave straight lines. The slope of the lines for the calculated lipid peroxidation was nearly equal with the experimental value. Similar results were obtained for oxidation of membrane proteins, except for band 3 oxidation. The values for the rate constants calculated from hemolysis curves seem to be accurate. The slope of the lines for the calculated rate constants for proteins was larger than the experimental value for band 3 oxidation, because band 3 oxidation is accompanied by aggregation or redistribution of band 3 proteins to form hemolytic holes. These results indicate that the competitive reaction model may be useful for analyzing radical-induced hemolysis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0003-9861
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
366
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
61-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Mechanism of free radical-induced hemolysis of human erythrocytes: comparison of calculated rate constants for hemolysis with experimental rate constants.
pubmed:affiliation
Faculty of Pharmaceutical Sciences, Tohoku University, Aobayama, Sendai, Aoba-Ku, 980-8578, Japan. yusato@mail.cc.tohoku.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't