Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-6-29
pubmed:abstractText
The morphology and behaviour of tissue cells when surrounded by a network of protein fibres, such as for a tissue-equivalent comprising cells entrapped in a type I collagen gel, is distinct from that when cells are cultured on a rigid surface, and physiologically relevant. The highly elongated and apparently bipolar morphology leads to a 'reversing' type of cell movement in gels, as opposed to a directionally persistent movement characteristic of highly spread, polar cells on surfaces. However, the hallmark of a tissue-equivalent is consolidation of the fibrillar network, or gel compaction, resulting from traction exerted by the cells. When the gel is mechanically constrained from compacting, alignment of the fibrils occurs, inducing cell alignment through a contact guidance response. In order to understand this 'self-organization' of tissue-equivalents, some relevant structural and mechanical properties of collagen gel are considered first, followed by a review of seminal studies of cell traction and tissue-equivalent compaction. Random cell migration in an isotropic gel is then discussed, including a modification of the persistent random walk model used to analyse cell migration on surfaces, followed by a review of contact guidance studies in gels with fibrils having defined alignment. With this background, observations of self-organization of mechanically constrained compacting tissue-equivalents are summarized and explained using a mechanical theory that relates traction-induced compaction to fibre alignment and consequent contact guidance, i.e. a strain-based rather than stress-based cell response to gel compaction. Data in support of this theory obtained from studies involving the controlled applied compression of tissue-equivalents are then presented. Finally, possible mechanisms of contact guidance are discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0067-8694
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
27-42
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Self-organization of tissue-equivalents: the nature and role of contact guidance.
pubmed:affiliation
Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Review