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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1999-6-17
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pubmed:abstractText |
MHC molecules are expressed at the surface of nucleated cells to present peptides to T cells. Structural information on MHC molecules has been gathered by x-ray crystallography techniques by using soluble proteins. Although relationships between MHC molecules and cell membranes have not been studied in detail, they are of critical importance for T cell recognition. Using a chemically modified lipid, we have been able to capture and orient histidine-tagged MHC molecules on lipid membranes. Surface plasmon resonance experiments show that the protein binds to the nickel lipid in a specific manner and in an oriented fashion, which allows T cell receptor binding. Similar lipid surfaces have been used to grow two-dimensional crystals and to determine the structure of a membrane-anchored murine H-2Kb MHC class I molecule. The docking of the crystallographic structure into the three-dimensional reconstructed structure derived from the two-dimensional crystals allows us to determine that the histidine tag is near the membrane surface and that the MHC molecule is in an upright position, exposing the peptide/alpha1-alpha2 domains toward the T cell.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-1331092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-1924358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-1998698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-2163898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-2254934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-3043536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-3260136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-3464971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-6289376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-6333027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-7306664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-7536435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-7602140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-7708669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-7793636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-7972089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8287475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8475596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8612131,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8637599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8642319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8643507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8712358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8758894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-8824178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9135137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9204133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9391114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9405151,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9469799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9506941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9597140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10318936-9653136
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5634-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10318936-Animals,
pubmed-meshheading:10318936-Crystallization,
pubmed-meshheading:10318936-Crystallography, X-Ray,
pubmed-meshheading:10318936-Diffusion,
pubmed-meshheading:10318936-H-2 Antigens,
pubmed-meshheading:10318936-Histidine,
pubmed-meshheading:10318936-Histocompatibility Antigens Class I,
pubmed-meshheading:10318936-Lipids,
pubmed-meshheading:10318936-Liposomes,
pubmed-meshheading:10318936-Membrane Proteins,
pubmed-meshheading:10318936-Mice,
pubmed-meshheading:10318936-Microscopy, Electron,
pubmed-meshheading:10318936-Models, Molecular,
pubmed-meshheading:10318936-Molecular Structure,
pubmed-meshheading:10318936-Surface Plasmon Resonance
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pubmed:year |
1999
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pubmed:articleTitle |
Structure and function of a membrane-bound murine MHC class I molecule.
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pubmed:affiliation |
Department of Immunology, The Scripps Research Institute, 10550, North Torrey Pines Road, La Jolla, CA 92037, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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